Recombinant Mouse Single-stranded DNA Cytosine Deaminase (Aicda)
Recombinant Mouse Single-stranded DNA Cytosine Deaminase (Aicda) to an enzyme synthesized using genetic engineering techniques from mouse sources. Aicda catalyzes the conversion of cytosine to uracil specifically in single-stranded DNA molecules. This enzyme is crucial for processes like antibody diversification and DNA repair in mammals, particularly in the context of adaptive immune responses.
Applications
Biotechnology
Aicda can be employed in various biotechnological applications, such as protein engineering, metabolic engineering, and the production of biotherapeutics. It enables precise modifications of DNA sequences, facilitating the development of novel bioproducts and biocatalysts.
Disease Modeling
Aicda-based gene editing techniques can be utilized to create animal models of human diseases, allowing researchers to study disease mechanisms, test therapeutic interventions, and develop new treatments.
Antibody Diversification
Aicda is essential for somatic hypermutation and class switch recombination in B cells, leading to the generation of diverse antibody repertoires. Recombinant Aicda can be employed to study and manipulate antibody diversity for therapeutic and research purposes.
Features
Research Utility
Aicda serves as a valuable tool for studying molecular mechanisms underlying DNA editing, immune function, and antibody generation. It enables researchers to investigate gene function, protein structure, and disease mechanisms
Safety
Recombinant versions of Aicda can be produced under controlled conditions to ensure purity and safety, minimizing the risk of off-target effects or unintended genetic modifications.
Efficiency
Aicda catalyzes the deamination of cytosine at a high efficiency rate, facilitating rapid and accurate DNA editing in vitro and in vivo.