AffiREC® Recombinant Mouse Leukemia inhibitory Factor

Research Area

Immunology.

Purity

> 98 % by SDS-PAGE and HPLC analyses..

Source

N/A.

Biological Activity

Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of Mouse M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures..

Synonyms

LIF, Mouse.

Molecular Weight

Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids..

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20..

Storage & Stability

-20ºC