AffiREC® Recombinant Human Tumor Necrosis Factor-alpha, Variant
CAT# AFG-BTL-17815
Size: 10 μg
AffiREC® Recombinant Human Tumor Necrosis Factor-alpha, Variant
Research Area
Immunology.
Purity
> 98 % by SDS-PAGE and HPLC analyses..
Source
N/A.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using Mouse L929 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D..Synonyms
TNF-α, DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF, Variant, Human.
Molecular Weight
Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 151 amino acids. Compared with the wild-type, rHuTNF-α Variant has an amino acid sequence (a.a.) deletion from a.a. 1-7, and the following a.a. substitutes Arg8, Lys9, Arg10 and Phe157 which is proven to have more activity and with less inflammatory side effect in vivo..
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0..
Storage & Stability
-20ºC