AffiREC® Recombinant Human Malic Enzyme 2, His
CAT# AFG-BTL-18317
Size: 5 μg
AffiREC® Recombinant Human Malic Enzyme 2, His
Research Area
N/A.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Source
N/A.
Biological Activity
Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975) . The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM.Synonyms
ME2, ODS1, Human.
Molecular Weight
Approximately 64.4 kDa, a single non-glycosylated polypeptide chain containing 573 amino acids with 6 × His at C-terminus.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Storage & Stability
-20ºC