AffiREC® Recombinant Human Malic Enzyme 2, His

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CAT# AFG-BTL-18317
Size: 5 μg

293.40 293.40000000000003 USD 293.40

293.40

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AffiREC® Recombinant Human Malic Enzyme 2, His

Research Area

N/A.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Source

N/A.

Biological Activity

Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975) . The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM.

Synonyms

ME2, ODS1, Human.

Molecular Weight

Approximately 64.4 kDa, a single non-glycosylated polypeptide chain containing 573 amino acids with 6 × His at C-terminus.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Storage & Stability

-20ºC