AffiREC® Recombinant Human LR3 Insulin-like Growth Factor-1

Research Area

Immunology.

Purity

>98 % by SDS-PAGE analyses.>90 % by RP-HPLC analyses.

Source

N/A.

Biological Activity

Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 IU/mg.Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

Synonyms

LR3 IGF-1, IGF, IGF-I, IGFI, MGF, Human.

Molecular Weight

Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2.

Storage & Stability

-20ºC