AffiREC® Clostridium Difficile Toxoid B

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CAT# AFG-NTG-0044
Size: 0.05 mg

1,012.49 1012.49 USD 1,012.49

1,012.49

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    Overview:

    Native toxin B purified from C. difficile strain VPI10463 (toxinotype 0), converted to inactive toxoid form

    Inactivation Method

    Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2µg/ml compared to active toxin lethal dose of 4pg/ml.

    Format

    Lyophilised. Bubbly/glassy residue due to lyophilisation in 5% sucrose to increase product stability.

    Buffer

    0.05M hepes, 0.15M NaCl, 5% sucrose.

    Vial Size

    0.05 mg

    Purity

    >95% pure by SDS-PAGE.

    Expected Molecular Weight

    N/A.

    Appearance

    Bubbly/glassy residue due to lyophilisation in 5% sucrose to increase product stability.

    Stain

    VPI10463.

    Tag

    N/A.

    Long-Term Storage Temperature (°C)

    +4.

    Short-Term Storage Temperature (°C)

    Lyophilised vial: +4°C Reconstituted liquid: +4°C.

    Shipping Conditions

    Ambient.

    Form

    Lyophilized powder  

    Buffer

    Tris/PBS-based buffer, 6% Trehalose, pH 8.0

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Shelf Life

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.