ADAR Recombinant Proteins 

ADAR (Adenosine Deaminase Acting on RNA) recombinant proteins are engineered versions of the enzymes that catalyze the conversion of adenosine (A) to inosine (I) in double-stranded RNA (dsRNA). This process, known as RNA editing, plays a critical role in regulating gene expression, maintaining RNA stability, and modulating immune responses. ADAR proteins are widely used in RNA biology, neurobiology, cancer research, and studies of innate immunity.

Content on ADAR Recombinant Protein

• Structure and Function
  • ADAR proteins have distinct domains:
    • dsRNA-binding Domains (dsRBDs): Facilitate the recognition and binding of dsRNA.
    • Deaminase Domain: Catalyzes the hydrolytic deamination of adenosine to inosine in RNA.
  • Key family members include:
    • ADAR1: Ubiquitously expressed, involved in immune regulation and viral RNA editing.
    • ADAR2: Predominantly expressed in the brain, critical for editing neuronal transcripts.
    • ADAR3: Brain-specific, with unclear catalytic activity but involved in RNA editing regulation.

Applications of ADAR Recombinant Protein

• RNA Editing Studies
  • Investigate A-to-I editing in coding and non-coding RNAs and its effects on transcriptome regulation.
  • Study the consequences of RNA editing on protein function and RNA stability.
• Immune Regulation and Innate Immunity
  • Explore ADAR1’s role in suppressing dsRNA-triggered innate immune responses and its involvement in autoimmune disorders.
  • Study ADAR-mediated editing of viral RNAs and its impact on antiviral immunity.
• Neurological Research
  • Analyze ADAR2’s role in editing key neuronal transcripts, such as GRIA2 (GluR-B), and its effects on neurotransmission and excitotoxicity.
  • Investigate ADAR dysfunction in neurological disorders like epilepsy, ALS, and schizophrenia.
• Cancer Research
  • Study aberrant ADAR activity and its effects on RNA editing in tumor progression and metastasis.
  • Explore ADAR1’s role in immune evasion by tumors through the suppression of cytosolic RNA sensing.

ADAR recombinant proteins are indispensable tools for advancing research in RNA biology, immunology, and therapeutic RNA editing, offering critical insights into the regulation of gene expression and potential treatment strategies for complex diseases.