Albumin Recombinant Proteins

Albumin, a major blood protein, maintains oncotic pressure and transports various substances. Recombinant albumin proteins, produced using biotechnological methods, ensure high purity and consistency for research and clinical applications.

Production Process

• Expression in Host Cells: Genes encoding human serum albumin (HSA) are inserted into host cells like Escherichia coli or Saccharomyces cerevisiae.
• Fermentation and Harvesting: Cells are cultured in bioreactors, harvested via centrifugation or filtration.
• Purification: Techniques like affinity and ion-exchange chromatography purify albumin, achieving >98% purity.
• Refolding and Formulation: Proteins are refolded to their native structure and formulated with stabilizers for storage.

Advantages

• Consistency and Safety: Ensures batch-to-batch consistency and eliminates pathogen contamination risk.
• Customization: Allows engineering of variants with specific properties.
• Scalability: Facilitates large-scale production.

Applications

• Clinical Use: Recombinant albumin is used in therapeutic applications, such as volume expansion, drug delivery, and as a component of biopharmaceutical formulations. It serves as an excipient in the formulation of various drugs, including vaccines, to enhance their stability and efficacy.
• Research Applications: In research, recombinant albumin is utilized as a standard in protein quantification assays, a blocking agent in immunoassays, and a carrier protein in drug delivery studies. Its high solubility and stability make it an ideal reagent for various biochemical and biophysical experiments.
• Diagnostics: Recombinant albumin is also employed in diagnostic assays, such as ELISAs and Western blots, where it serves as a blocking agent to prevent non-specific binding and enhance signal specificity.