ALKBH Recombinant Proteins

ALKBH (AlkB homolog) proteins are a family of Fe(II)/2-oxoglutarate-dependent dioxygenases involved in DNA and RNA repair by demethylating alkylated bases. Recombinant ALKBH proteins are produced using advanced biotechnological methods, ensuring high purity and consistency for research and therapeutic applications.

Production Process

• Gene Cloning: The gene encoding the ALKBH protein is cloned into an expression vector suitable for the chosen host cell system, typically E. coli, Saccharomyces cerevisiae, or mammalian cells.
• Expression in Host Cells: The recombinant vector is introduced into host cells, which are cultured under optimal conditions to express the ALKBH protein.
• Protein Purification: After cell lysis, ALKBH protein is purified using chromatographic techniques such as affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography to achieve high purity.
• Protein Refolding and Formulation: If necessary, the protein is refolded to its native conformation and formulated with appropriate stabilizers for storage and application.

Applications

• Research: Used to study DNA/RNA repair mechanisms, epigenetic modifications, and enzyme activity assays. ALKBH proteins are crucial in understanding their role in various biological processes and disease mechanisms.
• Drug Development: Employed in screening assays for inhibitors or modulators of ALKBH activity, which may lead to potential therapeutic agents for diseases involving DNA/RNA damage.
• Diagnostics: Utilized in diagnostic assays to investigate the presence and activity of ALKBH proteins in different biological samples.

Advantages

• Consistency and Safety: Recombinant production ensures batch-to-batch consistency and eliminates contamination risks.
• Customization: Allows for the production of protein variants with specific mutations or modifications for targeted studies.
• Scalability: Enables large-scale production to meet research and industrial demands.

Recombinant ALKBH proteins are essential tools in molecular biology and biomedical research, providing valuable insights into DNA/RNA repair and contributing to the development of novel therapeutic strategies.