ALKBH Recombinant Proteins 

ALKBH (AlkB homologs) recombinant proteins are engineered versions of proteins belonging to the ALKBH family, which are known for their roles as 2-oxoglutarate (2OG)-dependent dioxygenases. These proteins are involved in DNA and RNA repair mechanisms, specifically in demethylating alkylated nucleotides caused by oxidative stress or chemical exposure. ALKBH proteins have garnered significant attention for their importance in epigenetics, RNA modifications, and their potential as therapeutic targets in cancer and metabolic diseases.

Content:

Recombinant ALKBH proteins are produced using host expression systems such as E. coli, yeast, insect, or mammalian cells to ensure high-quality production. These proteins are available in various forms, including full-length and domain-specific constructs, to facilitate research and drug discovery.

Key features of ALKBH recombinant proteins:

• High Purity: Typically >95%, with low endotoxin levels (<1.0 EU/μg).
• Bioactivity: Retains enzymatic activity for demethylation of DNA or RNA substrates.
• Custom Isoforms: Includes wild-type, mutant, or tagged variants (e.g., His-tag, GST-tag) for specific applications.

Applications:

• Cancer Biology:
  • Investigation of ALKBH-mediated repair of alkylated DNA damage and its implications in chemotherapy resistance.
  • Target validation in oncology for inhibitors aimed at modulating ALKBH activity.
• Drug Discovery:
  • Screening for small-molecule inhibitors or activators of ALKBH enzymes.
  • Use in high-throughput assays to evaluate candidate compounds affecting 2OG-dependent dioxygenase activity.
• RNA Biology:
  • Analysis of mRNA stability and translation through ALKBH-mediated demethylation of RNA.
  • Investigation of ALKBH's role in regulating non-coding RNA and transcriptome dynamics.
• Structural and Biophysical Studies:
  • Crystallization and X-ray diffraction studies to determine ALKBH protein structures.
  • Kinetic assays to understand enzymatic mechanisms and cofactor interactions.

Recombinant ALKBH proteins serve as indispensable tools for understanding the intricate pathways of nucleic acid modification and repair, as well as advancing therapeutic strategies for targeting epigenetic and metabolic disorders.