Caspase Recombinant Proteins
Caspase recombinant proteins are engineered versions of caspases, a family of cysteine-aspartic proteases crucial for apoptosis (programmed cell death), inflammation, and cellular homeostasis. These proteases exist as inactive zymogens that, upon activation, cleave specific substrates to drive cellular signaling cascades. Recombinant production of caspase proteins allows for their use in research and drug development, particularly in studies on cell death mechanisms, cancer, neurodegeneration, and immune response.
Content:
Caspase recombinant proteins are typically produced in high-yield expression systems such as E. coli or insect cells to ensure proper folding and enzymatic activity. They are available in active or inactive forms and may include mutations or tags (e.g., His-tag, GST-tag) for enhanced utility.
Applications:
- Apoptosis Research:
- Investigation of intrinsic and extrinsic apoptosis pathways mediated by caspase activation.
- Assessment of caspase activation and cleavage events in cancer, neurodegeneration, and autoimmune diseases.
- Drug Discovery and Screening:
- High-throughput screening of caspase inhibitors or activators as therapeutic candidates.
- Evaluation of small molecules targeting caspase-mediated pathways in diseases like cancer and Alzheimer's.
- Structural and Mechanistic Studies:
- X-ray crystallography and cryo-EM studies of caspase proteins to understand substrate specificity and activation mechanisms.
- Analysis of caspase dimerization and zymogen-to-active protein conversion.
- Immune Response and Inflammation:
- Study of inflammatory caspases (e.g., Caspase-1, Caspase-4, Caspase-5) in pyroptosis and cytokine release.
- Investigation of inflammasome activation and its downstream signaling.
Recombinant caspase proteins are indispensable tools in the study of apoptosis, inflammation, and related cellular processes, offering insights into mechanisms underlying disease and enabling the development of targeted therapies.
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