CoA Recombinant Proteins
CoA recombinant proteins refer to engineered proteins involved in the metabolism and biosynthesis of coenzyme A (CoA), a vital cofactor in numerous biochemical pathways. CoA is integral to fatty acid metabolism, energy production, and the regulation of cellular processes through acetylation. Recombinant production of CoA-related enzymes, such as pantothenate kinase (PanK), CoA synthase (COASY), and acetyl-CoA synthetase (ACSS), enables detailed studies on CoA's biosynthesis and its role in metabolic regulation and disease.
Content:
Recombinant CoA proteins are typically expressed in bacterial (E. coli), yeast, or mammalian systems to ensure proper folding and enzymatic activity. These proteins are designed to retain their natural structure and activity, often incorporating tags (e.g., His-tag, GST-tag) for purification and application in assays.
Applications:
- Metabolic Pathway Research:
- Study of CoA biosynthesis and its regulation at key enzymatic steps, including PanK, COASY, and ACSS activity.
- Investigation of CoA's role in energy production and lipid metabolism.
- Drug Discovery:
- Screening of inhibitors targeting CoA biosynthetic enzymes for therapeutic applications in metabolic disorders, cancer, and microbial infections.
- Development of small molecules modulating CoA metabolism.
- Disease Modeling:
- Exploration of CoA metabolism in rare disorders such as Pantothenate Kinase-Associated Neurodegeneration (PKAN).
- Investigation of the role of CoA in cancer metabolism and mitochondrial dysfunction.
- Enzymatic Assays:
- Use in in vitro enzymatic activity assays to quantify substrate turnover, such as acetyl-CoA or malonyl-CoA formation.
- Kinetic studies to evaluate enzyme efficiency, cofactor requirements, and substrate specificity.
Recombinant CoA proteins provide critical tools for understanding the intricate roles of CoA in metabolism, offering valuable insights for therapeutic development in metabolic disorders, cancer, and infectious diseases.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|