DDX Recombinant Proteins 

DDX recombinant proteins are synthetic versions of the DEAD-box helicase family of proteins, expressed and purified using recombinant DNA technology. The DDX family is critical in RNA metabolism, including RNA splicing, ribosome biogenesis, translation initiation, and RNA decay. These proteins are characterized by their ATP-dependent RNA helicase activity and play roles in unwinding RNA secondary structures and facilitating RNA-protein interactions.

Production and Expression
  • Expressed in host systems such as E. coli, yeast, insect, or mammalian cells.
  • Engineered for high purity with minimal contaminants.
  • Often tagged (e.g., His-tag, GST-tag) for ease of purification and experimental applications.
Advantages
  • Batch consistency and high reproducibility.
  • Customizable with mutations for functional studies.
  • Absence of impurities associated with native extraction.
Applications of DDX Recombinant Proteins
  • Moléculaire Biology Research
    • Study RNA-protein interactions, RNA metabolism, and unwinding mechanisms.
    • Explore the role of DDX proteins in gene regulation and RNA structural dynamics.
  • Screening for Drug Targets
    • Act as substrates for high-throughput screening of small molecule inhibitors targeting helicase activity.
    • Study ATPase and helicase activity modulation for therapeutic development.
  • Structural Biology
    • Crystallization studies to determine the atomic structure of DDX proteins.
    • Explore conformational changes during ATP hydrolysis and RNA binding.
  • Biotechnological Applications
    • Incorporation into systems studying RNA-based processes like CRISPR/Cas technologies and ribonucleoprotein assembly.

DDX recombinant proteins are indispensable tools in understanding RNA biology, advancing therapeutic development, and providing insights into cellular RNA-dependent processes.