GPR Recombinant Proteins 

GPR (G protein-coupled receptors) recombinant proteins are engineered biomolecules designed to mimic naturally occurring GPRs, integral membrane proteins involved in cellular signal transduction. These recombinant proteins are synthesized using advanced expression systems such as mammalian, bacterial, or insect cell lines to ensure proper folding, post-translational modifications, and functionality. GPRs are critical in mediating responses to extracellular stimuli, including hormones, neurotransmitters, and environmental signals.

Content of GPR Recombinant Proteins

• Structure:
  GPR recombinant proteins retain the characteristic seven-transmembrane α-helical structure essential for ligand binding and intracellular signaling. Structural integrity is verified through methods such as circular dichroism (CD) spectroscopy and X-ray crystallography.
• Stability and Activity:
  GPR recombinant proteins are optimized for thermal stability and functional integrity to support downstream applications such as binding assays and structural studies.
• Validation:
  Quality assurance includes functional assays such as ligand-binding affinity tests, GTPγS binding assays, and receptor activation assays.

Applications of GPR Recombinant Proteins

• Functional Assays:
  • Used in cell-free systems to study receptor-ligand interactions.
  • Employed in assays measuring second-messenger signaling pathways, including cyclic AMP (cAMP), calcium flux, or phosphatidylinositol turnover.
• Structural Biology:
  Crystallized forms of GPR recombinant proteins facilitate structural analysis through techniques like cryo-electron microscopy, elucidating receptor conformational changes upon ligand binding.
• Pathophysiological Research:
  • Critical for understanding GPR involvement in various diseases, including cancer, cardiovascular disorders, and metabolic syndromes.
  • Enable functional characterization of GPR mutations implicated in genetic disorders.
• Biosensor Development:
  GPR recombinant proteins are immobilized on biosensor platforms for real-time detection of ligands, making them indispensable in diagnostic and monitoring applications.

By providing precise control over protein expression and purification, GPR recombinant proteins ensure reproducibility and reliability in experimental and clinical applications. Their role in elucidating GPR-mediated signaling mechanisms continues to advance biomedical research and therapeutic innovation.