KDM Recombinant Proteins

Lysine demethylases (KDMs) are enzymes that remove methyl groups from lysine residues on histones, playing crucial roles in the regulation of gene expression and epigenetic modifications. KDMs are involved in various biological processes and are implicated in several diseases, including cancer. Recombinant KDM proteins are produced using advanced biotechnological methods to ensure high purity and consistency, facilitating research into their functions and therapeutic potential.

Production Process

• Gene Cloning: The gene encoding the specific KDM is cloned into an expression vector suitable for the chosen host system, typically Escherichia coli, insect cells, or mammalian cells.
• Expression in Host Cells: The recombinant vector is introduced into host cells, which are cultured under optimal conditions to express the KDM protein.
• Protein Purification: After cell lysis, KDM proteins are purified using chromatographic techniques such as affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography to achieve high purity.
• Protein Refolding and Formulation: If necessary, proteins are refolded to attain their functional conformation and formulated with stabilizers for storage and application.

Applications

• Research:
  • Epigenetic Studies: Used to investigate the role of KDMs in histone modification, gene expression, and chromatin dynamics.
  • Functional Assays: Employed in assays to study the enzymatic activity of KDMs and their interactions with substrates and other proteins.
• Drug Development:
  • Screening: Utilized in high-throughput screening assays to identify inhibitors or modulators of KDM activity, which could serve as potential therapeutic agents.
  • Mechanistic Studies: Helps in understanding the molecular mechanisms of diseases related to dysregulated KDM activity, aiding the development of targeted therapies.
• Diagnostics:
  • Biomarker Discovery: Used in assays to detect changes in KDM expression or activity as biomarkers for diseases such as cancer and neurodegenerative disorders.

Advantages

• Consistency and Safety: Recombinant production ensures high purity, batch-to-batch consistency, and eliminates contamination risks.
• Customization: Allows for the production of specific KDM variants, including those with specific mutations or modifications, for detailed mechanistic studies.
• Scalability: Facilitates large-scale production to meet research and industrial demands.

Recombinant KDM proteins are essential tools in the study of epigenetic regulation and gene expression. Their use in research, drug development, and diagnostics helps advance our understanding of the biological roles of KDMs and their potential as therapeutic targets.