KDM Recombinant Proteins 

KDM (lysine-specific demethylase) recombinant proteins are laboratory-engineered forms of KDM enzymes, a family of histone demethylases critical for the removal of methyl groups from lysine residues on histones. These proteins play a significant role in epigenetic regulation by dynamically modifying chromatin structure and gene expression. KDM recombinant proteins are produced using advanced expression systems to ensure activity, structural integrity, and compatibility with various applications in biochemical and epigenetic research.

Content of KDM Recombinant Proteins

• Expression Systems:
  • Bacterial Systems: Used for simplified production of truncated or catalytically active domains.
  • Mammalian Systems: Essential for producing full-length proteins with proper folding and post-translational modifications.
  • Insect Systems: Provide high yields and maintain functional enzyme characteristics.
• Validation and Purification:
  • Purification: Achieved via affinity chromatography using tags such as His or GST.
  • Functional Validation: Assessed through demethylase activity assays using histone peptide substrates or full-length nucleosomes.
• Active and Mutant Variants:
  • Active forms for functional studies.
  • Catalytically inactive mutants for control experiments and structural studies.

Applications of KDM Recombinant Proteins

• Epigenetic Research:
  • Study the specific roles of KDMs in histone demethylation and chromatin dynamics.
  • Investigate the interplay between histone modifications (e.g., methylation and acetylation).
• Drug Discovery and Development:
  • Screening small molecules or inhibitors targeting KDMs as potential epigenetic drugs, particularly for cancer and neurodegenerative diseases.
  • Characterizing drug specificity and mechanism of action using recombinant proteins.
• Biochemical Assays:
  • Used in in vitro demethylase assays to quantify enzymatic activity and substrate specificity.
  • Employed in studying enzyme kinetics, substrate binding, and cofactor requirements.
• Structural Biology:
  • Recombinant KDM proteins facilitate crystallization and analysis of enzyme-substrate complexes, aiding in the development of structure-based inhibitors.

By enabling high-throughput assays and detailed mechanistic studies, KDM recombinant proteins are indispensable tools in advancing our understanding of histone methylation dynamics and their implications in health and disease.