KLH Recombinant Proteins

Keyhole Limpet Hemocyanin (KLH) Recombinant Proteins are crucial in biomedical research due to their strong immunogenic properties. Derived from the hemolymph of Megathura crenulata, KLH proteins are used primarily as carrier proteins in antibody production, vaccine development, and immunotoxicology studies.

Technical Details

Structure:

• High-molecular-weight glycoprotein (~8 MDa) existing as a didecamer.
• Composed of subunits KLH1 and KLH2, each with multiple copper-binding sites.

Production:

• Recombinant KLH is produced using genetic engineering in hosts like E. coli or yeast.
• Ensures high yield, consistent quality, and reduced variability.

Applications:

• Immunogenic Carrier Protein:
  • Enhances immunogenicity of haptens and peptides.
  • Used in monoclonal and polyclonal antibody production.
• Vaccine Development:
  • Acts as an immunostimulant and adjuvant.
  • Used in cancer immunotherapy and infectious disease vaccines.
• Immunotoxicology:
  • Assesses immune function and immunotoxicity.
  • Serves as a biomarker in immunological assays.
• Neurobiology:
  • Investigates neurodegenerative disease mechanisms.

Analytical Methods:

• SDS-PAGE/Western Blot: For molecular weight and purity.
• HPLC: For size and aggregation state.
• Mass Spectrometry: For structural analysis.
• ELISA: For immunogenicity assessment.