KLH Recombinant Proteins 

KLH (Keyhole Limpet Hemocyanin) recombinant proteins are engineered forms of the hemocyanin molecule derived from the keyhole limpet (Megathura crenulata). KLH is a large, oxygen-carrying glycoprotein with immunostimulatory properties widely used in immunological research and vaccine development. Recombinant KLH proteins are produced using advanced expression systems to ensure batch consistency, structural integrity, and immunogenic activity.

Content of KLH Recombinant Proteins

• Expression Systems:
  • Eukaryotic Systems: Used for generating recombinant KLH with proper glycosylation and folding.
  • Bacterial Systems: Suitable for producing truncated or functionally active KLH domains for specific applications.
• Purification:
  • Recombinant KLH proteins are purified using techniques like affinity chromatography and gel filtration to ensure high purity and structural consistency.
• Validation:
  • Verified for immunogenicity through antigen-specific immune assays.
  • Characterized for structural fidelity using analytical methods such as SDS-PAGE and mass spectrometry.

Applications of KLH Recombinant Proteins

• Vaccine Development:
  • Employed as an adjuvant or carrier protein in therapeutic vaccines for cancer, autoimmune diseases, and infectious diseases.
  • Aids in enhancing antigen presentation and promoting robust T-cell responses.
• Diagnostic Applications:
  • Utilized in the development of ELISA kits for detecting specific antibodies.
  • Recombinant KLH can be conjugated with diagnostic markers for serological assays.
• Therapeutic Applications:
  • KLH-based immunotherapies are under investigation for treating cancers, such as melanoma, and chronic conditions like bladder cancer.

By providing a reliable and customizable platform for immunological studies, KLH recombinant proteins have become invaluable tools in immunotherapy research, vaccine development, and diagnostics.