MITF Recombinant Proteins 

MITF (Microphthalmia-associated Transcription Factor) recombinant proteins are engineered forms of the MITF protein, a key transcription factor involved in the regulation of melanocyte development, pigmentation, and cell survival. MITF belongs to the basic helix-loop-helix leucine zipper (bHLH-Zip) family of transcription factors and plays a crucial role in various biological processes, including melanogenesis and oncogenesis. These recombinant proteins are produced in heterologous expression systems to ensure structural fidelity and functional activity for research and therapeutic applications.

Content of MITF Recombinant Proteins

Structural Features:

• Contains a DNA-binding bHLH-Zip domain that enables specific interaction with E-box sequences in target gene promoters.
• Functional regions for transcriptional activation and interaction with cofactors like SOX10 and CREB.

Expression Systems:

• Bacterial Systems: Used for producing truncated or domain-specific MITF proteins for biochemical studies.
• Mammalian Systems: Essential for generating full-length MITF with post-translational modifications (e.g., phosphorylation and acetylation).

Purification:

• Produced as highly pure proteins using affinity tags (e.g., His-tag, GST-tag) and chromatographic techniques.
• Designed for compatibility with downstream functional and structural assays.

Applications of MITF Recombinant Proteins

• Developmental Research:
  • Study MITF's role in neural crest cell lineage specification and its interaction with SOX10 and PAX3 during embryogenesis.
• Drug Discovery:
  • Screen small molecules or peptide inhibitors targeting MITF to modulate its activity in diseases like melanoma or vitiligo.
  • Assess the efficacy of therapies designed to enhance pigmentation for cosmetic or medical applications.
• Epigenetic and Post-Translational Studies:
  • Evaluate the impact of phosphorylation, acetylation, or ubiquitination on MITF's function and stability.
  • Study the epigenetic regulation of MITF expression in response to external stimuli.

By enabling detailed investigation of MITF's biological roles and regulatory mechanisms, recombinant MITF proteins are essential tools in melanocyte biology, cancer research, and transcriptional regulation studies.