MTHFD Recombinant Proteins 

MTHFD (Methylenetetrahydrofolate Dehydrogenase) recombinant proteins are engineered forms of the MTHFD enzyme family, which play critical roles in one-carbon metabolism and folate-mediated cellular processes. These enzymes are involved in the interconversion of tetrahydrofolate (THF) derivatives, facilitating the synthesis of nucleotides, amino acids, and other biomolecules. Recombinant MTHFD proteins are produced using advanced expression systems to retain their structural and enzymatic integrity, making them indispensable in metabolic research and therapeutic applications.

Content of MTHFD Recombinant Proteins

• Validation:
  • Purity and activity are validated using assays for enzymatic activity (e.g., NADP/NAD reduction assays) and structural integrity.
  • Additional characterization using techniques like mass spectrometry and X-ray crystallography.
• Post-Translational Modifications:
  • Includes phosphorylation, crucial for regulating MTHFD2 in metabolic signaling pathways.

Applications of MTHFD Recombinant Proteins

• Drug Discovery:
  • High-throughput screening of small molecules that inhibit MTHFD activity for therapeutic applications in cancer, metabolic disorders, and mitochondrial diseases.
  • Test potential drug candidates targeting MTHFD enzymes in models of folate metabolism dysfunction.
• Structural Biology:
  • Recombinant MTHFD proteins are used to determine the 3D structure of the enzyme to understand substrate binding and catalytic mechanisms.
  • Facilitate structure-based drug design through crystallization and cryo-EM studies.
• Therapeutic Development:
  • Used to investigate MTHFD-associated metabolic pathways in disorders such as neural tube defects and cardiovascular diseases.
  • Enable studies on mitochondrial one-carbon metabolism and its role in developmental biology.
• Diagnostic and Biomarker Research:
  • Aid in developing assays to measure MTHFD activity or expression as biomarkers in cancer and metabolic disorders.

MTHFD recombinant proteins are essential tools for advancing research in cellular metabolism, mitochondrial biology, and therapeutic targeting of metabolic diseases and cancer.