MUC Recombinant Proteins
MUC (Mucin) recombinant proteins are engineered forms of mucins, a family of high-molecular-weight glycoproteins that play essential roles in maintaining epithelial barrier function and mediating cell signaling. Mucins are heavily glycosylated proteins expressed on epithelial surfaces or secreted into mucus, contributing to immune defense, hydration, and lubrication.
Content of MUC Recombinant Proteins
- Expression Systems:
- Mammalian Systems: Commonly used for producing full-length or glycosylated mucins to ensure native-like post-translational modifications.
- Insect Cell Systems: Suitable for expressing domains or regions of mucins for structural studies.
- Bacterial Systems: Employed for producing truncated or unglycosylated mucin fragments for antibody generation or biochemical studies.
- Domain-Specific Variants:
- Recombinant proteins may represent the extracellular tandem repeat domains, which are heavily glycosylated, or intracellular cytoplasmic domains involved in signal transduction.
- Post-Translational Modifications:
- Include extensive O-glycosylation, critical for biological function and immune evasion.
- Validation:
- Characterized using glycan profiling, mass spectrometry, and functional assays to confirm structural and functional integrity.
Applications of MUC Recombinant Proteins
- Therapeutic Development:
- Aid in developing mucin-based cancer vaccines targeting tumor-associated glycoforms of mucins.
- Support the design of monoclonal antibodies and small-molecule inhibitors for clinical applications.
- Mucosal Barrier Research:
- Study the role of secreted mucins in protecting epithelial surfaces in the gastrointestinal, respiratory, and reproductive tracts.
- Investigate mucin dysregulation in diseases such as inflammatory bowel disease (IBD), cystic fibrosis, and chronic obstructive pulmonary disease (COPD).
- Biomarker Discovery:
- MUC16 (CA125) and MUC1 (CA15-3) are widely used as biomarkers for ovarian and breast cancer detection, respectively.
- Recombinant mucins enable the identification and validation of novel diagnostic markers.
MUC recombinant proteins are indispensable tools for studying epithelial biology, tumor microenvironment interactions, and immune modulation, with broad implications in cancer, infectious diseases, and chronic inflammatory conditions.
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