MUC Recombinant Proteins
Mucin (MUC) Recombinant Proteins are vital in the study of mucins, which are high molecular weight glycoproteins predominantly found in mucus secretions. Mucins play essential roles in protecting and lubricating the epithelial surfaces of the respiratory, gastrointestinal, and urogenital tracts. They are also involved in cell signaling and modulating immune responses.
Technical Details
Structure:
- Mucins are characterized by their extensive glycosylation, which provides a gel-like consistency to mucus.
- They have a protein backbone rich in serine, threonine, and proline residues, with O-linked glycans attached to these residues.
Production:
- MUC recombinant proteins are produced using recombinant DNA technology in systems such as E. coli, yeast, insect, or mammalian cells.
- The recombinant production ensures high yield, purity, and consistent quality, facilitating detailed functional and structural studies.
Applications:
- Disease Research:
- Used to study the role of mucins in diseases such as cancer, cystic fibrosis, and inflammatory bowel diseases.
- Helps in understanding mucin overexpression, aberrant glycosylation, and their contribution to disease pathogenesis.
- Drug Development:
- Target for the development of therapeutic agents aimed at modulating mucin expression and function.
- Screening and development of inhibitors or monoclonal antibodies against specific mucin domains.
- Immunology:
- Investigating the role of mucins in modulating immune responses and their interaction with pathogens.
- Studying mucin's role in barrier function and host defense mechanisms.
- Glycobiology:
- Understanding the structure-function relationship of mucin glycosylation.
- Analyzing the impact of glycan modifications on mucin properties and interactions.
Analytical Methods:
- Mass Spectrometry: For detailed glycan analysis and characterization of post-translational modifications.
- NMR Spectroscopy and X-ray Crystallography: For structural analysis of mucin domains and their interactions.
- Western Blotting and ELISA: For detection and quantification of mucins in biological samples.
- Surface Plasmon Resonance (SPR): To study mucin interactions with other biomolecules.
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