Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) Recombinant Proteins are crucial for studying the NF-κB signaling pathway, which plays a vital role in regulating immune response, inflammation, cell proliferation, and survival. NF-κB is a protein complex that controls the transcription of DNA and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, and bacterial or viral antigens.
Technical Details
Structure:
- NF-κB is composed of dimers formed by members of the Rel protein family, which includes p65 (RelA), RelB, c-Rel, p50, and p52.
- The p65/p50 heterodimer is the most common and extensively studied form of NF-κB.
- NF-κB proteins contain Rel homology domains (RHD) responsible for DNA binding, dimerization, and interaction with inhibitory IκB proteins.
Production:
- NF-κB recombinant proteins are produced using recombinant DNA technology in systems such as E. coli, yeast, insect, or mammalian cells.
- Recombinant production ensures high purity and functional integrity of the proteins for biochemical and cellular studies.
Applications:
- Signal Transduction Research:
- Used to study the NF-κB signaling pathway and its regulation.
- Helps in understanding the mechanisms of NF-κB activation, translocation, and DNA binding.
- Disease Research:
- NF-κB is involved in various diseases, including cancer, autoimmune disorders, chronic inflammation, and neurodegenerative diseases.
- Studying NF-κB function and regulation provides insights into the pathogenesis of these conditions and potential therapeutic targets.
- Drug Development:
- Target for the development of anti-inflammatory and anti-cancer drugs.
- Screening and characterization of small molecule inhibitors, peptides, and monoclonal antibodies that modulate NF-κB activity.
- Transcriptional Regulation:
- Investigating the role of NF-κB in regulating the expression of genes involved in immune response, apoptosis, and cell proliferation.
- Functional assays to study NF-κB-mediated transcriptional activation.
Analytical Methods:
- Electrophoretic Mobility Shift Assay (EMSA): To study DNA-binding activity of NF-κB.
- Chromatin Immunoprecipitation (ChIP): To identify NF-κB target genes and binding sites in vivo.
- Western Blotting: For detection and quantification of NF-κB subunits and post-translational modifications.
- Luciferase Reporter Assays: To measure NF-κB transcriptional activity.
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