NFKB Recombinant Proteins 

NFKB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) recombinant proteins are engineered forms of the transcription factors that constitute the NF-κB family. NF-κB proteins are critical regulators of immune responses, inflammation, cell proliferation, and apoptosis. The family includes various subunits, such as RelA (p65), RelB, c-Rel, p50, and p52, which form homo- or heterodimers to modulate gene expression. 

Content of NFKB Recombinant Proteins

• Expression Systems:
  • Bacterial Systems: Widely used for producing DNA-binding domains or full-length subunits with high yields.
  • Mammalian Systems: Essential for generating proteins with post-translational modifications (e.g., phosphorylation).
• Post-Translational Modifications (PTMs):
  • Phosphorylation: Critical for activation and nuclear translocation.
  • Acetylation and ubiquitination: Regulate transcriptional activity and protein stability.
• Validation:
  • Verified using DNA-binding assays, electrophoretic mobility shift assays (EMSAs), and luciferase reporter assays.
  • Purity confirmed through SDS-PAGE, mass spectrometry, and Western blotting.

Applications of NFKB Recombinant Proteins

• Cancer Research:
  • Explore the dysregulation of NF-κB pathways in cancers, particularly in promoting cell survival, angiogenesis, and metastasis.
  • Screen inhibitors targeting NF-κB signaling for anti-cancer therapies.
• Drug Discovery and Screening:
  • Recombinant NF-κB proteins are used in high-throughput screens for small-molecule inhibitors of NF-κB activation.
  • Test compounds modulating IκB kinase (IKK) activity or NF-κB nuclear translocation.
• Gene Regulation Studies:
  • Analyze NF-κB binding to specific κB sites on DNA using in vitro binding assays.
  • Characterize the role of NF-κB in regulating gene networks through chromatin immunoprecipitation (ChIP) assays.

NFKB recombinant proteins are essential tools for unraveling the complex mechanisms of NF-κB signaling, advancing research in immunology, oncology, and drug development. Their versatility supports detailed studies of this pivotal transcription factor family in health and disease.