SCP Recombinant Proteins Overview

SCP (Sterol Carrier Protein) Recombinant Proteins are vital in the study of lipid metabolism and intracellular cholesterol transport. SCP is involved in the transport of sterols and other lipids between intracellular compartments, playing a crucial role in maintaining cellular lipid homeostasis.

Technical Details

Structure:

• SCPs are small cytoplasmic proteins with a highly conserved sterol-binding domain.
• They exhibit a helix-loop-helix motif essential for binding and transporting sterols.

Production:

• SCP recombinant proteins are produced using recombinant DNA technology in systems such as E. coli, yeast, insect, or mammalian cells.
• Recombinant production ensures high purity and functional integrity, suitable for biochemical and structural studies.

Applications:

• Lipid Metabolism Research:
  • Used to study the mechanisms of intracellular cholesterol and lipid transport.
  • Helps in understanding the role of SCPs in lipid homeostasis and metabolic regulation.
• Disease Research:
  • SCPs are implicated in metabolic disorders, including obesity, diabetes, and cardiovascular diseases.
  • Investigating SCP function provides insights into the molecular basis of these conditions and potential therapeutic targets.
• Drug Development:
  • Targeting SCPs for the development of therapeutics aimed at modulating lipid metabolism.
  • Screening and characterization of small molecule inhibitors or activators of SCPs.
• Structural Biology:
  • Studying the three-dimensional structure of SCPs to understand their sterol-binding mechanism.
  • Investigating protein-lipid interactions and the conformational changes associated with lipid binding.

Analytical Methods:

• X-ray Crystallography and NMR: For structural determination and analysis of SCPs.
• Lipid Binding Assays: To measure the binding affinity and specificity of SCPs for different sterols and lipids.
• Fluorescence Spectroscopy: To study protein-lipid interactions and dynamics.
• Western Blotting and ELISA: For detection and quantification of SCPs in biological samples.