SNAP Recombinant Proteins 

SNAP recombinant proteins are engineered proteins derived from the SNAP-tag, a self-labeling protein tag commonly used in molecular and cell biology. The SNAP-tag is a 20 kDa mutant of O6-alkylguanine-DNA alkyltransferase (AGT), designed to covalently bind to benzylguanine (BG) derivatives. This unique feature enables specific and efficient labeling of SNAP-tagged fusion proteins with various probes, such as fluorescent dyes, biotin, or other functional groups.

Content of SNAP Recombinant Proteins

SNAP recombinant proteins are available in various formats, including:

• Tagged SNAP Proteins: Fused with specific protein tags like His-tag or GST for ease of purification and immobilization.
• SNAP-Protein Fusions: Functional domains or full-length proteins linked to SNAP for targeted applications.
• Label-Ready SNAP Proteins: Modified to be compatible with specific benzylguanine-based substrates for visualization or bioconjugation.

Applications of SNAP Recombinant Proteins

SNAP recombinant proteins are utilized in a variety of applications:

• Drug Discovery:
  • High-throughput screening assays to identify inhibitors or modulators of target proteins fused to SNAP.
• Biochemical Assays:
  • Quantitative detection of protein expression levels and activity using SNAP-tag conjugates.
• Biosensor Development:
  • Construction of SNAP-tag-based biosensors for real-time molecular detection.
• Super-Resolution Microscopy:
  • Enables precise protein localization studies by labeling SNAP-tagged proteins with high-performance dyes.

SNAP recombinant proteins are extensively employed in molecular biology, cell biology, and biochemistry, offering unparalleled flexibility and specificity for cutting-edge research applications.