TSH Recombinant Proteins 

Thyroid-Stimulating Hormone (TSH) recombinant proteins are bioengineered versions of TSH, a glycoprotein hormone secreted by the anterior pituitary gland. TSH plays a crucial role in regulating thyroid function by stimulating the synthesis and release of thyroid hormones, triiodothyronine (T3) and thyroxine (T4), via interaction with the TSH receptor (TSHR) on thyroid follicular cells.

Content of TSH Recombinant Proteins

• Host Systems:
  • Mammalian Expression: Preferred for ensuring accurate post-translational modifications, particularly glycosylation.
  • E. coli or Yeast Systems: Used for producing non-glycosylated forms or subunit-specific variants.
• Purity and Quality:
  • Purified to ≥95% by HPLC or SDS-PAGE to eliminate contaminants.
  • Low endotoxin levels (<0.01 EU/µg) for sensitive applications.
• Variants and Tags:
  • Wild-type TSH or modified versions to study receptor-binding properties.
  • Tagged forms (e.g., His-tag or FLAG-tag) for ease of detection and immobilization.

Applications of TSH Recombinant Proteins

• Thyroid Function Research:
  • Investigating the mechanisms of TSH-mediated stimulation of the thyroid gland.
  • Studying the regulation of T3 and T4 synthesis in health and thyroid disorders such as hypothyroidism and hyperthyroidism.
• Signal Transduction Studies:
  • Exploring TSHR-mediated activation of cyclic AMP (cAMP) and other intracellular pathways.
  • Analyzing downstream effects of TSH signaling in thyroid follicular cells.
• Drug Discovery and Screening:
  • Evaluating the efficacy of small molecules, peptides, or antibodies targeting the TSHR.
  • Identifying potential therapeutics for Graves' disease, thyroid cancers, or TSHR-related pathologies.
• Diagnostic Assay Development:
  • Used in immunoassays and ELISAs for the quantitative detection of TSH in clinical samples.
  • Production of TSH calibrators and controls for thyroid function testing.

TSH recombinant proteins are indispensable tools for advancing our understanding of thyroid physiology, developing targeted therapies, and improving diagnostic methodologies for thyroid-related disorders.