AlCDA Recombinant Proteins
Activation-Induced Cytidine Deaminase (AICDA), also known as AID, is an enzyme critical for somatic hypermutation (SHM) and class switch recombination (CSR) in immunoglobulin genes. These processes are essential for antibody diversity in adaptive immunity.
Content
- Structure and Function:
- AICDA is a member of the APOBEC family, catalyzing cytidine deamination to uracil in single-stranded DNA.
- Plays a central role in antibody diversification by introducing mutations and recombination events in immunoglobulin genes.
- Expression Systems:
- Bacterial Systems (e.g., E. coli): Efficient for producing functional AICDA for biochemical studies.
- Mammalian Systems: Suitable for producing post-translationally modified AICDA for functional assays.
- Insect Cells: Offer high yields and native folding for structural studies.
Applications
- Cancer Research:
- Exploring AICDA’s involvement in genomic instability in B-cell lymphomas.
- Investigating AICDA-mediated off-target effects in oncogenesis.
- Genome Editing and DNA Repair Studies:
- Using AICDA for targeted mutagenesis in single-stranded DNA regions.
- Studying the interplay between AICDA and DNA repair pathways such as mismatch repair and base excision repair.
- Drug Discovery:
- Screening inhibitors of AICDA for therapeutic intervention in autoimmunity and cancer.
- Evaluating compounds that modulate AICDA activity to influence antibody production.
AICDA recombinant proteins are indispensable tools for advancing immunological research, cancer biology, and therapeutic development, offering unique insights into the enzyme’s role in antibody diversification and genomic integrity.
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