ASGPR Recombinant Proteins
Asialoglycoprotein Receptor (ASGPR) is a transmembrane receptor predominantly expressed on hepatocytes. It plays a critical role in endocytosis by mediating the clearance of asialoglycoproteins from the bloodstream. The receptor recognizes terminal galactose and N-acetylgalactosamine residues on glycoproteins, binding with high specificity.
Content of ASGPR Recombinant Protein
- Structure:
- Typically includes the extracellular domain of ASGPR to facilitate ligand-binding studies.
- Commonly expressed in HEK293, CHO, or E. coli systems, with mammalian systems providing glycosylated forms for native-like functionality.
- May be available with affinity tags (e.g., His, FLAG) for easy purification and detection.
- Physical and Biochemical Properties:
- Molecular Weight: Approximately 30–50 kDa, depending on glycosylation status.
- Stability: Supplied in lyophilized or liquid form for extended storage and activity.
- Solubility: Soluble in physiological buffers for use in functional assays.
- Endotoxin Levels: Maintained below 1 EU/µg to ensure compatibility with sensitive systems.
Applications of ASGPR Recombinant Protein
- Hepatocyte Research:
- Used to study ASGPR's role in hepatocyte-specific endocytosis.
- Enables exploration of receptor-mediated uptake mechanisms and liver function.
- Disease Research:
- Helps elucidate ASGPR's involvement in liver diseases such as hepatocellular carcinoma, liver fibrosis, and autoimmune liver disorders.
- Used to study autoimmune antibodies targeting ASGPR in autoimmune hepatitis.
- Structural and Functional Studies:
- Facilitates research on the molecular interactions between ASGPR and its ligands.
- Supports structural biology studies, including crystallography and cryo-EM, to understand receptor-ligand binding.
Recombinant ASGPR proteins are valuable tools for advancing our understanding of liver-specific receptor biology, designing hepatocyte-targeted therapies, and investigating ASGPR-related diseases. Their high purity and functional integrity ensure reliability in basic and translational research applications.
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