CCHFV Recombinant Proteins 

CCHFV (Crimean-Congo Hemorrhagic Fever Virus) recombinant proteins are engineered versions of viral antigens that are critical for understanding the pathogenesis of CCHFV, a tick-borne virus responsible for severe hemorrhagic fever in humans. These proteins, derived from structural (e.g., glycoproteins, nucleoproteins) and non-structural (e.g., NSm, NSs) viral components, are widely used in vaccine development, diagnostic assays, and research into viral-host interactions.

Content on CCHFV Recombinant Proteins

• Key Antigenic Proteins
  • Glycoproteins (Gn and Gc): Involved in viral entry and membrane fusion, making them critical targets for neutralizing antibodies.
  • Nucleoprotein (N): Plays a role in RNA encapsidation and is the primary immunodominant antigen in CCHFV diagnostics.
  • L Protein (RNA-dependent RNA polymerase): Essential for viral replication.
  • Non-structural Proteins (NSm, NSs): Contribute to viral replication and evasion of host immune responses.
• Expression Systems
  • Prokaryotic Systems (e.g., E. coli): Commonly used for producing small, non-glycosylated antigens such as the nucleoprotein.
  • Eukaryotic Systems (e.g., insect or mammalian cells): Necessary for glycoproteins that require post-translational modifications to retain their native structure and function.

Applications of CCHFV Recombinant Proteins

• Vaccine Development
  • Evaluate immunogenicity and protective efficacy of viral glycoproteins and nucleoproteins.
  • Serve as antigens in subunit vaccine platforms targeting CCHFV.
• Diagnostic Assay Development
  • Serological Assays: Recombinant proteins, especially nucleoprotein and glycoproteins, are used as antigens in ELISA, immunofluorescence, or lateral flow assays to detect CCHFV-specific antibodies.
  • Molecular Diagnostics: Serve as positive controls or calibrators in PCR-based assays.
• Viral Pathogenesis Studies
  • Investigate the roles of structural and non-structural proteins in viral entry, replication, and immune evasion.
  • Study mechanisms of host immune response modulation by CCHFV antigens.

CCHFV recombinant proteins are invaluable tools for advancing research into the biology of the virus, developing effective vaccines, and improving diagnostic capabilities to combat this severe and often fatal disease.