CEACAM Recombinant Proteins 

CEACAM recombinant proteins refer to synthetic proteins derived from members of the Carcinoembryonic Antigen-Related Cell Adhesion Molecule (CEACAM) family, produced using recombinant DNA technology. These glycoproteins are involved in various cellular processes, including cell adhesion, signaling, immune response modulation, and microbial interactions. Recombinant CEACAM proteins are critical tools for research in oncology, immunology, and infectious diseases.

Technical Content
  • Production:
    • Gene Cloning: CEACAM genes are cloned into expression vectors optimized for their specific requirements (e.g., glycosylation sites).
    • Expression Systems:
      • Mammalian cells (e.g., HEK293, CHO) for proper glycosylation and folding.
      • Insect cell systems for multi-domain CEACAM proteins.
    • Purification: Employs affinity chromatography (e.g., His-tag) followed by size-exclusion chromatography to ensure high purity.
  • Key Features:
    • Structural Integrity: Retains functional domains, such as the Ig-like extracellular domains necessary for cell adhesion and immune interactions.
    • Batch-to-Batch Consistency: High reproducibility across production lots.
    • Customizable: Variants can be engineered to mimic disease-specific mutations or domain deletions.
  • Validation:
    • Structural validation via methods like circular dichroism (CD) or X-ray crystallography.
    • Functional assays include ligand-binding studies and immune cell activation tests.
Applications of CEACAM Recombinant Proteins
  • Infectious Disease Research:
    • Pathogen Interaction: CEACAM proteins serve as receptors for certain bacteria (e.g., Neisseria species, Helicobacter pylori) and viruses, making them essential for studying microbial adhesion and invasion mechanisms.
    • Development of anti-adhesion therapies targeting bacterial interactions.
  • Diagnostics:
    • CEACAM5 recombinant proteins are used as antigens in diagnostic assays for cancer detection.
    • CEACAM1 and CEACAM8 proteins are explored for immune monitoring applications.
  • Cell Signaling Studies:
    • Recombinant CEACAM proteins enable detailed studies of their role in cell-cell adhesion, intracellular signaling, and immune cell interactions.
Conclusion

CEACAM recombinant proteins are indispensable in advancing research in cancer, immunology, and infectious diseases. Their high specificity and functionality make them critical tools for understanding disease mechanisms, developing diagnostics, and innovating therapeutic approaches.