COL6A Recombinant Proteins 

Collagen VI (COL6A) is a critical extracellular matrix protein composed of three α chains, typically α1, α2, and α3, which are encoded by the COL6A1, COL6A2, and COL6A3 genes. COL6A is a non-fibrillar collagen with a unique triple-helix structure. It plays an essential role in tissue organization and cellular function by providing structural support and facilitating cell adhesion, migration, and differentiation. Recombinant COL6A proteins are engineered through recombinant DNA technology, expressing these proteins in systems like E. coli, yeast, or mammalian cells.

Content: 

Recombinant COL6A proteins consist of the α chains that form the COL6A trimeric structure. These proteins are engineered with high precision to match the native forms, including post-translational modifications like glycosylation. The recombinant proteins are purified through affinity chromatography or other advanced purification techniques to obtain high-quality preparations suitable for a range of applications.

Applications:

  • Cell Culture Studies:
    • Recombinant COL6A proteins are used in cell adhesion and migration assays. They are critical for understanding the interaction between cells and the extracellular matrix in various tissue types, including muscle, skin, and cartilage.
    • They are utilized to study cellular responses to matrix proteins, such as cell spreading, proliferation, and differentiation, making them vital in regenerative medicine, wound healing, and tissue engineering.
  • Disease Modeling:
    • Recombinant COL6A proteins are essential for creating in vitro models of collagen VI-related diseases such as Ullrich congenital muscular dystrophy (UCMD) and Bethlem myopathy. By understanding how the recombinant protein behaves in these models, researchers can gain insights into disease pathogenesis and potential therapeutic approaches.
  • Matrix Formation Studies:
    • COL6A proteins contribute to the formation of extracellular matrix scaffolds. These recombinant proteins help researchers investigate how collagen VI interacts with other matrix components like fibronectin, laminins, and integrins. This is crucial for studying tissue architecture in development and pathology.
  • Therapeutic Development:
    • Recombinant COL6A proteins can be explored in the development of therapeutic treatments for collagen VI-associated disorders. They provide the foundation for the creation of cell-based therapies or gene therapies aimed at restoring normal collagen VI function in affected tissues.

By utilizing COL6A recombinant proteins in these varied applications, researchers can enhance our understanding of extracellular matrix biology and develop new therapeutic strategies for related diseases.