CSN Recombinant Proteins 

CSN (COP9 Signalosome) recombinant protein is a multiprotein complex that plays a crucial regulatory role in cellular processes such as protein degradation, transcriptional regulation, and signal transduction. The CSN complex primarily mediates the deneddylation of cullin-RING E3 ubiquitin ligases (CRLs), thereby controlling their activity and stability. CSN proteins are essential for understanding cellular homeostasis, cell cycle progression, and stress responses.

Content of CSN Recombinant Protein

CSN recombinant proteins are produced using advanced expression systems (e.g., bacterial, insect, or mammalian cells) to ensure proper folding and functional integrity. They are typically expressed as individual subunits or as a reconstituted complex, depending on the experimental requirements. These proteins may include affinity tags (e.g., His-tag or GST-tag) for purification and detection. Functional recombinant CSN retains its ability to modulate CRLs and other associated pathways.

Applications of CSN Recombinant Protein

• Ubiquitin-Proteasome System Research
  CSN proteins are studied to understand their role in regulating CRL-mediated ubiquitination and proteasomal degradation.
• Cell Cycle Studies
  Essential for exploring how the CSN complex influences cell cycle transitions through the regulation of cyclin-dependent processes.
• Cancer Biology
  CSN components are implicated in tumorigenesis due to their role in regulating protein turnover, making them targets for drug development in oncology.
• Drug Discovery
  CSN recombinant proteins serve as tools for high-throughput screening of inhibitors targeting the neddylation or deneddylation pathways.
• Stress Response and Signaling
  CSN’s involvement in stress-related signaling pathways, such as responses to DNA damage or oxidative stress, is a focus of cellular and molecular research.
• Structural Biology
  Recombinant CSN proteins are used for crystallographic and spectroscopic studies to elucidate the structural organization and interaction networks within the CSN complex.

CSN recombinant protein is a critical tool for investigating the complex regulatory mechanisms of protein homeostasis and signal transduction, with applications in basic research and therapeutic development.