DEGP Recombinant Proteins 

DEGP, also known as HtrA (high-temperature requirement A) protease, is a serine protease with dual protease and chaperone activities. It plays a critical role in protein quality control, especially under stress conditions, by degrading misfolded or damaged proteins. DEGP is predominantly studied in prokaryotes like Escherichia coli but has homologs in eukaryotes, including HtrA1, HtrA2/Omi, and HtrA3, which are implicated in cellular homeostasis, apoptosis, and neurodegenerative diseases.

Content of DEGP Recombinant Protein

• Production:
  • Recombinant DEGP proteins are expressed in bacterial systems such as E. coli for efficient production and functional activity.
  • Purified using affinity chromatography (e.g., His-tag or GST-tag systems) to achieve high purity.
• Variants:
  • Wild-type DEGP for studying native activity and structure.
  • Mutant forms with point mutations in the protease or chaperone domain to assess functional mechanisms.
  • Truncated proteins for domain-specific studies.
• Specifications:
  • Purity: >95% as confirmed by SDS-PAGE or mass spectrometry.
  • Activity: Validated through substrate cleavage assays and thermal stress tests.
  • Storage: Typically provided in buffer systems with stabilizers to maintain activity at -80°C.

Applications of DEGP Recombinant Protein

• Protein Quality Control Studies:
  • Used to analyze the role of DEGP in identifying and degrading misfolded proteins under heat stress or oxidative conditions.
  • Investigate its dual protease and chaperone activity to understand cellular stress management mechanisms.
• Structural Biology:
  • Supports X-ray crystallography and cryo-EM studies to determine the structure of DEGP in active and inactive states.
  • Structural insights help elucidate substrate recognition and enzymatic cleavage mechanisms.
• Drug Discovery and Screening:
  • Screening of small-molecule inhibitors targeting DEGP's protease activity, particularly for applications in infectious disease research.
  • Evaluating modulators of DEGP activity in stress-related disorders and bacterial pathogenicity.

DEGP recombinant protein is a critical resource for understanding protein quality control, enzymatic regulation, and cellular stress responses. Its versatility in applications, ranging from basic research to therapeutic development, underscores its importance in molecular and biomedical sciences.