DNM Recombinant Proteins 

DNM (Dynamin) recombinant proteins are engineered versions of dynamins, a family of large GTPase proteins essential for membrane remodeling processes. Dynamins play a crucial role in vesicle trafficking, endocytosis, organelle division, and cytoskeletal dynamics. Recombinant DNM proteins are widely used in studying their molecular mechanisms, structural biology, and involvement in various diseases, including neurodegenerative disorders and cancer.

Content on DNM Recombinant Proteins

• Structure and Function
  • Dynamins consist of the following domains:
    • GTPase Domain: Hydrolyzes GTP to provide energy for membrane fission.
    • Middle Domain and GTPase Effector Domain (GED): Facilitate oligomerization and conformational changes during function.
    • PH (Pleckstrin Homology) Domain: Binds to phospholipids on the membrane.
    • PRD (Proline-Rich Domain): Interacts with SH3-domain-containing proteins.
  • Key isoforms include:
    • DNM1: Predominantly involved in neuronal endocytosis.
    • DNM2: Ubiquitously expressed and critical for clathrin-mediated endocytosis.
    • DNM3: Plays specialized roles in synaptic vesicle recycling and cardiac function.
• Expression Systems
  • Prokaryotic Systems (e.g., E. coli): Suitable for producing individual domains or truncated versions.
  • Eukaryotic Systems (e.g., insect or mammalian cells): Essential for expressing full-length DNM proteins with post-translational modifications.

Applications of DNM Recombinant Proteins

• Endocytosis and Vesicle Trafficking Research
  • Study the role of dynamins in clathrin-mediated and caveolin-mediated endocytosis.
  • Investigate vesicle scission and recycling processes in various cell types.
• Membrane Dynamics Studies
  • Analyze the molecular mechanisms of membrane remodeling and fission.
  • Explore interactions between dynamins and lipid bilayers or cytoskeletal elements.
• Structural Biology
  • Perform crystallographic and cryo-EM studies to elucidate the oligomeric structure of dynamins.
  • Analyze conformational changes during GTP hydrolysis and membrane fission.

DNM recombinant proteins are indispensable tools for advancing research in cellular trafficking, membrane dynamics, and disease pathophysiology, offering critical insights for therapeutic development and structural studies.