EGFR Recombinant Proteins
EGFR recombinant proteins are synthetically produced forms of the Epidermal Growth Factor Receptor (EGFR), a transmembrane tyrosine kinase receptor that is part of the ErbB family. EGFR plays a critical role in regulating cell proliferation, survival, and differentiation. Dysregulation of EGFR signaling is implicated in various cancers, including lung, colorectal, and head-and-neck cancers.
Technical Content
- Expression Systems:
- Mammalian cells (e.g., HEK293, CHO): Preferred for glycosylated and full-length proteins.
- Baculovirus-infected insect cells: Used for high-yield production of functional proteins.
- E. coli: Suitable for non-glycosylated domains like the kinase domain.
- Purification and Quality Control:
- Purified via affinity chromatography using tags such as His-tag or GST-tag, followed by size-exclusion chromatography.
- Quality validated by:
- SDS-PAGE and Western blotting for purity and size.
- Functional assays (e.g., kinase activity or ligand binding assays).
- Mass spectrometry for structural integrity.
- Variants and Mutations:
- Recombinant EGFR proteins include cancer-relevant mutations like EGFRvIII (a constitutively active mutant) and T790M (associated with drug resistance).
Applications of EGFR Recombinant Proteins
- Cancer Research:
- Study the role of EGFR in tumor growth, metastasis, and angiogenesis.
- Investigate the impact of EGFR mutations on signaling pathways and drug resistance mechanisms.
- Drug Development and Screening:
- Used to screen small molecule inhibitors targeting the tyrosine kinase domain (e.g., erlotinib, gefitinib).
- Facilitate the evaluation of therapeutic antibodies (e.g., cetuximab) that block ligand binding to the extracellular domain.
- Structural Biology:
- Crystallographic and cryo-EM studies of EGFR domains provide insights into receptor activation and inhibitor binding.
- Structural data are critical for designing next-generation EGFR-targeted therapies.
EGFR recombinant proteins are essential for advancing cancer research, drug discovery, and therapeutic development. Their high quality and precise engineering make them indispensable tools for studying EGFR-mediated signaling, evaluating inhibitors, and designing strategies to overcome drug resistance.
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