EPCAM Recombinant Proteins 

Epithelial Cell Adhesion Molecule (EPCAM), also known as CD326, is a transmembrane glycoprotein primarily expressed on epithelial cells. It plays a key role in cell adhesion, signaling, proliferation, and differentiation. EPCAM is highly upregulated in various cancers and is widely studied as a cancer stem cell marker and therapeutic target.

Content of EPCAM Recombinant Proteins

• Production:
  • Recombinant EPCAM proteins are produced using mammalian or insect cell systems to preserve native folding and post-translational modifications.
  • Protein purity is ensured through affinity chromatography (e.g., His-tag or Fc-tag purification).
• Variants:
  • Full-length EPCAM proteins for studying its overall function and receptor-ligand interactions.
  • Extracellular domain constructs for antibody development or ligand-binding studies.
  • Mutated or truncated forms to assess domain-specific functions.
• Specifications:
  • Purity: >95% as assessed by SDS-PAGE or SEC.
  • Biological activity: Validated through binding or cell-based assays.
  • Stability: Supplied in buffered solutions optimized for storage at -80°C.

Applications of EPCAM Recombinant Proteins

• Cancer Research:
  • EPCAM is extensively studied as a biomarker for epithelial cancers, including colorectal, breast, and ovarian cancers.
  • Recombinant EPCAM proteins are used in tumor biology studies to understand its role in cell adhesion and metastasis.
• Structural Studies:
  • Structural analysis of EPCAM using recombinant proteins provides insights into its interaction with ligands and co-receptors.
  • Facilitates understanding of its conformational changes during adhesion or signal transduction.
• Diagnostics:
  • Used in the development of diagnostic assays for detecting EPCAM-positive circulating tumor cells (CTCs) in liquid biopsy applications.
  • Enables standardization of assays for cancer progression and prognosis.

EPCAM recombinant proteins are indispensable for studying its diverse biological functions, particularly in cancer and regenerative medicine. Their applications span basic research, diagnostics, and therapeutic development, making them vital tools in translational and clinical research efforts.