Filoviruses Recombinant Proteins 

Filoviruses recombinant proteins are synthetic proteins derived from members of the Filoviridae family, which includes deadly viruses such as Ebola virus (EBOV) and Marburg virus (MARV). These proteins, which mimic native viral antigens, are critical for understanding the molecular mechanisms of infection, immune response, and pathogenesis. Recombinant technology allows the production of key structural and non-structural proteins, including glycoproteins (GP), nucleoproteins (NP), and VP40 matrix proteins, with high purity and functionality.

Technical Content

• Expression Systems:
  • Mammalian Expression Systems (e.g., HEK293, CHO cells): Used for glycoproteins requiring proper folding and post-translational modifications such as glycosylation.
  • E. coli Expression Systems: Suitable for non-glycosylated proteins like NP or truncated domains.
  • Insect Cell Systems: Frequently employed for complex, multi-domain proteins.
• Purification and Validation:
  • Purification via affinity chromatography (e.g., His-tag, GST-tag) to achieve >90% purity.
  • Verified for functionality using assays such as ELISA, Western blot, and flow cytometry.
  • Structural integrity confirmed using techniques like SDS-PAGE and circular dichroism (CD).

Applications of Filoviruses Recombinant Proteins

• Vaccine Development:
  • Glycoproteins (GP) are primary antigens used in the development of vaccines, including virus-like particles (VLPs) and subunit vaccines.
  • Aid in evaluating immune responses and the development of neutralizing antibodies.
• Therapeutic Research:
  • Used to screen and evaluate antiviral drugs targeting viral entry, replication, or immune evasion mechanisms.
  • VP35 and VP24 proteins serve as tools to study host immune modulation and potential therapeutic interventions.
• Serological and Diagnostic Assays:
  • Recombinant proteins such as GP and NP are used as antigens in ELISA and immunoblot assays to detect filovirus-specific antibodies in human and animal samples.
  • Development of rapid diagnostic tests for filoviruses during outbreaks.
• Basic Virology Research:
  • Enable structural and functional studies of filovirus replication, assembly, and host interaction mechanisms.
  • GP and VP40 are key tools for studying virus-host cell entry and budding processes.

Filoviruses recombinant proteins are indispensable for advancing research into the biology, immunology, and therapeutic targeting of these highly pathogenic viruses. Their precision engineering ensures reliable use in vaccine development, drug discovery, diagnostic assay design, and structural studies, significantly contributing to global efforts to combat filovirus outbreaks.