HBA Recombinant Proteins 

HBA (Hemoglobin subunit alpha) recombinant proteins are engineered versions of the alpha subunit of hemoglobin, a critical protein involved in oxygen transport within erythrocytes. These recombinant proteins are used in studies related to hemoglobinopathies, oxygen binding and release, and red blood cell physiology.

Content on HBA Recombinant Proteins

• Structure and Function
  • The HBA protein is a globin chain that forms part of the hemoglobin tetramer, typically composed of two alpha (HBA) and two beta (HBB) subunits.
  • It binds heme groups, enabling reversible oxygen binding for transport to tissues and facilitating carbon dioxide removal.
• Expression Systems
  • Prokaryotic Systems (e.g., E. coli): Cost-effective for generating soluble HBA chains.
  • Eukaryotic Systems (e.g., yeast, insect, or mammalian cells): Produce functionally active HBA with proper folding and post-translational modifications.

Applications of HBA Recombinant Proteins

• Hemoglobinopathies Research
  • Study genetic mutations in the HBA gene that lead to conditions such as alpha-thalassemia and hemoglobin H disease.
  • Analyze the structural and functional impacts of these mutations on oxygen affinity and stability.
• Diagnostics and Therapeutics
  • Biomarker Discovery: Investigate HBA levels and post-translational modifications as potential biomarkers for hemolytic anemia and other red blood cell disorders.
  • Therapeutics: Use recombinant HBA to design oxygen carriers for transfusion medicine or synthetic blood substitutes.
• Oxygen Transport Studies
  • Examine oxygen binding and release mechanisms under physiological and pathological conditions.
  • Use recombinant HBA to model interactions with ligands like carbon monoxide and nitric oxide.

HBA recombinant proteins are vital for advancing hemoglobin-related research, offering tools for understanding oxygen transport, hemoglobinopathies, and the development of innovative diagnostics and therapeutics.