HCV Recombinant Proteins
HCV recombinant proteins are laboratory-produced proteins derived from the Hepatitis C virus (HCV). These proteins represent key viral components such as structural (Core, E1, E2) and non-structural proteins (NS3, NS4, NS5). HCV recombinant proteins are critical tools in the study of viral infection mechanisms, immunology, vaccine research, and diagnostic assay development.
Content on HCV Recombinant Proteins
HCV recombinant proteins are produced in advanced expression systems, including E. coli, yeast, and mammalian cells, to ensure proper folding and biological functionality. Key characteristics include:
- Protein Variants: Core protein, E1/E2 envelope glycoproteins, NS3 protease, NS4A/B, and NS5A/B.
- Purity: Typically >95%, verified using SDS-PAGE and HPLC.
- Expression Systems:
- Mammalian cells for glycosylated envelope proteins (E1, E2).
- E. coli for non-structural proteins and fragments.
Applications of HCV Recombinant Proteins
- Serological Diagnostics:
- Widely used in ELISA and CLIA assays for detecting anti-HCV antibodies in patient samples.
- Vaccine Research:
- Serves as a basis for developing vaccines targeting HCV structural and non-structural proteins.
- Drug Discovery:
- Facilitates screening of antiviral drugs targeting HCV enzymes, such as NS3 protease and NS5B polymerase.
- Immunological Studies:
- Helps analyze T-cell and B-cell responses to HCV proteins in infected or vaccinated individuals.
HCV recombinant proteins are essential tools for advancing Hepatitis C research, facilitating diagnostic development, therapeutic discovery, and vaccine production.
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