HEV Recombinant Proteins 

HEV recombinant proteins are engineered proteins derived from the Hepatitis E virus (HEV), a significant cause of acute viral hepatitis worldwide. These proteins, often including structural (capsid ORF2) and non-structural (ORF3, ORF1) components, are essential tools for studying HEV pathogenesis, immune responses, vaccine development, and diagnostic applications. Recombinant production ensures high-quality, reproducible proteins suitable for research and commercial uses.

Content on HEV Recombinant Proteins

HEV recombinant proteins are typically produced in expression systems like E. coli, yeast, or mammalian cells, ensuring functional integrity and biological activity. Key features include:

• Variants:
  • Capsid Protein (ORF2): The most immunogenic and widely studied.
  • ORF3 Protein: Associated with virus-host interactions.
  • Non-structural proteins: For studying HEV replication.
• Purity: Typically >95%, validated using SDS-PAGE and HPLC analysis.

Applications of HEV Recombinant Proteins

• Serological Diagnostics:
  • Used as antigens in ELISA and Western blot assays to detect anti-HEV IgG and IgM antibodies in clinical samples.
• Vaccine Development:
  • Plays a central role in developing recombinant protein-based HEV vaccines.
• Drug Screening:
  • Facilitates screening and characterization of antiviral agents targeting HEV replication and infection mechanisms.
• Immunological Studies:
  • Analyzes host immune responses to HEV, particularly T-cell and B-cell interactions with HEV antigens.

HEV recombinant proteins are indispensable tools in virology, immunology, and diagnostics. Their high-quality production and retained biological properties make them essential for advancing research on HEV pathogenesis, vaccine development, and therapeutic interventions.