HMGB Recombinant Proteins 

HMGB (High Mobility Group Box) recombinant proteins are non-histone chromatin-binding proteins essential for DNA-related processes such as transcription, replication, and repair. HMGB proteins, including HMGB1, HMGB2, and HMGB3, play pivotal roles in chromatin remodeling and act as architectural factors to facilitate protein-DNA interactions. Beyond their nuclear functions, extracellular HMGB proteins serve as damage-associated molecular patterns (DAMPs) that mediate inflammatory and immune responses, making them critical targets in disease research.

Content of HMGB Recombinant Protein

HMGB recombinant proteins are expressed in bacterial, yeast, or mammalian systems to ensure functional integrity and proper folding. These proteins are often tagged (e.g., His-tag or GST-tag) to enable streamlined purification and detection. HMGB proteins retain conserved DNA-binding domains (HMG boxes) responsible for their interactions with chromatin and other DNA substrates. Recombinant HMGB1, the most studied isoform, is often used in research related to inflammation and cancer.

Applications of HMGB Recombinant Protein

• Cancer Research
  HMGB proteins are involved in tumor progression, invasion, and metastasis. Recombinant HMGB1 is studied as a biomarker and therapeutic target.
• Immunology and Inflammation
  Extracellular HMGB1 acts as a pro-inflammatory cytokine and is used in studies of autoimmune diseases, sepsis, and inflammation.
• DNA Repair and Chromatin Remodeling
  HMGB proteins are used to investigate their roles in facilitating DNA repair processes and chromatin dynamics.
• Drug Discovery
  HMGB recombinant proteins serve as targets for developing inhibitors or modulators of HMGB-mediated pathways in diseases like cancer, neurodegeneration, and inflammatory disorders.
• Structural Biology
  Recombinant HMGB proteins are used for crystallography and NMR studies to elucidate their interaction with DNA and other chromatin-associated factors.
• Neurodegenerative Disease Research
  HMGB1 is implicated in neuroinflammation and cell death in diseases such as Alzheimer's and Parkinson's, making it a focus of neurobiology studies.

HMGB recombinant proteins are indispensable tools in molecular biology, with broad applications in understanding chromatin biology, immune responses, and pathophysiological processes.