HRP Recombinant Proteins
HRP (Horseradish Peroxidase) recombinant protein is an enzyme widely used in biochemistry and molecular biology due to its ability to catalyze the oxidation of various substrates in the presence of hydrogen peroxide (H₂O₂), resulting in detectable colorimetric, fluorometric, or chemiluminescent signals. Recombinant HRP mimics the properties of native HRP, offering high enzymatic activity and stability while ensuring batch-to-batch consistency for research and diagnostic applications.
Content of HRP Recombinant Protein
HRP recombinant protein is expressed in bacterial, yeast, or mammalian systems, ensuring efficient folding and enzymatic activity. It retains critical functional domains required for peroxidase activity, including the heme-binding site. HRP is often modified or tagged (e.g., His-tag) for specific experimental needs, such as immobilization or conjugation to antibodies, proteins, or nucleic acids.
Applications of HRP Recombinant Protein
- Immunoassays
- ELISA, Western Blot, and Immunohistochemistry: HRP is conjugated to secondary antibodies for signal generation and amplification.
- Molecular Diagnostics
- Used in diagnostic kits to detect biomarkers, pathogens, or genetic material with high sensitivity.
- Enzyme Kinetics
- Investigate the catalytic activity of HRP in oxidizing substrates like TMB (tetramethylbenzidine), DAB (3,3′-diaminobenzidine), or luminol.
- Signal Amplification in Biosensors
- HRP is integrated into biosensor platforms to amplify signals in detecting small molecules, proteins, or DNA.
- Drug Discovery and Screening
- Recombinant HRP is used in high-throughput assays to identify modulators of enzyme activity.
- Oxidative Stress Studies
- Used to model and study reactive oxygen species (ROS)-mediated processes.
HRP recombinant protein is indispensable in research and diagnostic fields, providing robust, reproducible, and versatile tools for enzyme-based detection and signal amplification.
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