HSP Recombinant Proteins
HSP (Heat Shock Protein) recombinant proteins are engineered proteins that belong to a highly conserved family of molecular chaperones. These proteins are critical in maintaining cellular proteostasis by assisting in protein folding, preventing aggregation, and facilitating the refolding or degradation of misfolded proteins.
Content on HSP Recombinant Proteins
- Structure and Function
- HSP27 (Small HSPs): Prevent aggregation of misfolded proteins under stress conditions.
- HSP60: Facilitates protein folding within mitochondria and bacterial systems.
- HSP70: Assists in ATP-dependent folding of nascent polypeptides and refolding of denatured proteins.
- HSP90: Plays a critical role in stabilizing and activating client proteins, including steroid hormone receptors and kinases.
- HSP100: Participates in protein disaggregation under extreme stress conditions.
- Expression Systems
- Prokaryotic Systems (e.g., E. coli): Efficient for producing recombinant HSPs with active chaperone domains.
- Eukaryotic Systems (e.g., insect or mammalian cells): Preserve post-translational modifications critical for complex chaperone functions.
Applications of HSP Recombinant Proteins
- Cancer Research
- HSP90 Inhibition: Study the role of HSP90 in stabilizing oncogenic client proteins and as a therapeutic target in cancer.
- HSP70 and Apoptosis: Explore how HSP70 modulates apoptosis and cellular survival in tumors.
- Neurodegenerative Diseases
- Examine the role of HSPs in preventing aggregation of pathogenic proteins, such as tau or α-synuclein, in diseases like Alzheimer’s and Parkinson’s.
- Develop therapeutic strategies targeting HSPs to enhance proteostasis in neurodegenerative disorders.
- Stress Response Research
- Study cellular responses to heat shock, oxidative stress, or other proteotoxic conditions.
- Use HSPs as biomarkers for cellular stress in toxicological assessments.
HSP recombinant proteins are indispensable for advancing our understanding of protein homeostasis and stress biology, with wide-ranging applications in research, diagnostics, and therapeutics.
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