IFN Recombinant Proteins
Interferons (IFNs) are a family of cytokines that play essential roles in immune response regulation, antiviral defense, and cell signaling. Classified into three main types—Type I (e.g., IFN-α, IFN-β), Type II (e.g., IFN-γ), and Type III (e.g., IFN-λ)—IFNs modulate various biological processes, including inflammation, apoptosis, and pathogen elimination.
Content of IFN Recombinant Proteins
- Production:
- Expressed in E. coli, yeast, or mammalian cells to ensure proper folding and biological activity.
- Purification is performed using affinity chromatography, such as His-tag purification, or ion-exchange chromatography.
- Variants:
- Individual isoforms of IFN-α (e.g., IFN-α2a, IFN-α2b) for functional studies.
- Full-length IFN-β, IFN-γ, and IFN-λ for research and therapeutic purposes.
- Mutant or pegylated forms for enhanced stability and extended half-life in therapeutic research.
- Specifications:
- Purity: >95% as confirmed by SDS-PAGE or HPLC.
- Activity: Bioactivity validated through antiviral or immunomodulatory assays.
- Storage: Supplied in buffers optimized for stability at -80°C.
Applications of IFN Recombinant Proteins
- Immunological Research:
- Study the signaling pathways activated by IFN-stimulated genes (ISGs), such as JAK-STAT signaling.
- Investigate the immunomodulatory effects of IFNs on T cells, B cells, and dendritic cells.
- Antiviral Studies:
- IFN recombinant proteins are used to evaluate their efficacy against viral pathogens, including hepatitis viruses, influenza, and SARS-CoV-2.
- Screening antiviral agents that modulate IFN response pathways.
- Cancer Research:
- Type I and Type II IFNs are studied for their antitumor effects, including inducing apoptosis, inhibiting angiogenesis, and enhancing immune surveillance.
- Used in assays to test immunotherapeutic approaches like immune checkpoint inhibitors.
Recombinant IFN proteins are invaluable in advancing our understanding of immune responses and in the development of therapies for viral infections, autoimmune diseases, and cancer. Their versatility in research and therapeutic applications makes them essential tools in immunology and biomedical sciences.
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