IRF Recombinant Proteins 

IRF (Interferon Regulatory Factor) recombinant proteins represent engineered versions of the IRF family of transcription factors, which play a pivotal role in immune regulation, antiviral responses, and inflammation. These proteins are involved in the expression of interferons (IFNs) and IFN-stimulated genes (ISGs) and are critical in immune surveillance, pathogen recognition, and cancer immunology.

Content on IRF Recombinant Proteins

• Structure and Function
  • IRFs are characterized by:
    • DNA-binding Domain (DBD): Recognizes specific IFN-stimulated response elements (ISREs) in target gene promoters.
    • Transactivation Domain (TAD): Activates transcription upon signaling.
  • Key family members and their roles include:
    • IRF3/IRF7: Regulate type I IFN responses during viral infections.
    • IRF5: Mediates inflammatory cytokine production.
    • IRF4/IRF8: Critical for immune cell differentiation and function.
    • IRF1/IRF2: Regulate immune surveillance and tumor suppression.
• Expression Systems
  • Prokaryotic Systems (e.g., E. coli): Efficient for producing truncated or functional domains of IRFs.
  • Eukaryotic Systems (e.g., mammalian or insect cells): Required for full-length IRFs with post-translational modifications, such as phosphorylation.

Applications of IRF Recombinant Proteins

• Immune Regulation Studies
  • Investigate the role of IRF1 and IRF8 in immune cell differentiation and macrophage activation.
  • Analyze IRF5-driven inflammatory cytokine production in autoimmune diseases.
• Cancer Research
  • Explore tumor suppressor roles of IRF1 and IRF8 in immune surveillance and apoptosis.
  • Study IRF dysregulation in cancers to identify therapeutic targets.
• Inflammatory and Autoimmune Diseases
  • Study the involvement of IRF5 and IRF7 in conditions like lupus, rheumatoid arthritis, and psoriasis.
  • Develop inhibitors targeting IRF-mediated inflammatory pathways.

IRF recombinant proteins are indispensable for advancing research in immunology, virology, and cancer biology, providing key insights into transcriptional regulation and therapeutic targeting of immune pathways.