JAK Recombinant Proteins 

Janus Kinase (JAK) proteins are non-receptor tyrosine kinases integral to signal transduction pathways, particularly the JAK-STAT (Signal Transducer and Activator of Transcription) pathway. JAK proteins, including JAK1, JAK2, JAK3, and TYK2, play crucial roles in mediating cellular responses to cytokines and growth factors, influencing processes such as immune response, hematopoiesis, and cellular growth.

Content of JAK Recombinant Proteins
  • Production:
    JAK recombinant proteins are expressed in systems such as E. coli, insect cells, or mammalian cells to ensure proper folding and post-translational modifications. The proteins are purified using affinity chromatography or ion-exchange chromatography for high specificity.
  • Variants:
    • Full-length JAK proteins with catalytic activity.
    • Truncated or domain-specific variants (e.g., SH2 domain or kinase domain).
    • Mutant forms for structure-function analysis.
  • Specifications:
    • Purity: Typically >95% by SDS-PAGE.
    • Activity: Confirmed through kinase assays.
    • Storage: Formulated for stability at -80°C, often in buffers such as PBS with cryoprotectants.
Applications of JAK Recombinant Proteins
  • Signal Transduction Studies:
    JAK proteins are essential tools in studying the JAK-STAT pathway, enabling the identification of downstream effectors and regulatory mechanisms.
  • Structural Biology:
    • Recombinant JAK proteins are used for crystallization and structural studies to understand conformational changes during activation or inhibition.
    • Provide insights into binding sites for small molecules and peptides.
  • Disease Modeling:
    • Aid in creating in vitro models for disorders such as JAK2-driven polycythemia vera or JAK3-associated severe combined immunodeficiency (SCID).
    • Support mechanistic studies on cytokine signaling dysregulation in autoimmune conditions.
  • Custom Research Applications:
    • Tools for validating antibody specificity in immunoprecipitation or Western blot experiments.
    • Studying protein-protein interactions and phosphorylation events.

JAK recombinant proteins are indispensable in elucidating the molecular dynamics of signaling pathways and in the development of targeted therapies. Their high purity and functionality make them valuable resources in fundamental research, drug discovery, and diagnostic assay development.