KGP Recombinant Proteins 

Kgp (Lysine-specific gingipain) is a cysteine protease produced by Porphyromonas gingivalis, a key pathogen associated with periodontitis. Kgp is involved in degrading host proteins, disrupting immune responses, and promoting bacterial colonization. Its lysine-specific proteolytic activity is critical for bacterial virulence, making it an important target for periodontal disease research.

Content of Kgp Recombinant Protein

• Production:
  • Expressed in E. coli, yeast, or insect cells to maintain enzymatic activity and structural integrity.
  • Purified using affinity chromatography methods such as His-tag or GST-tag systems.
• Variants:
  • Full-length Kgp for studying its proteolytic activity and host interactions.
  • Domain-specific constructs for structural or binding studies (e.g., catalytic or adhesion domains).
  • Mutant forms to investigate the role of specific residues in protease activity.
• Specifications:
  • Purity: >90% confirmed by SDS-PAGE or HPLC.
  • Activity: Verified through substrate cleavage assays using lysine-specific substrates.
  • Storage: Supplied in buffer systems optimized for stability at -80°C.

Applications of Kgp Recombinant Protein

• Periodontal Disease Research:
  • Investigate the role of Kgp in the pathogenesis of periodontal infections.
  • Study its interaction with host proteins like fibrinogen and complement components to understand immune evasion strategies.
• Drug Discovery and Screening:
  • High-throughput screening of inhibitors targeting Kgp activity for therapeutic development.
  • Evaluate the efficacy of small molecules or peptides in blocking Kgp-mediated proteolysis.
• Vaccine Development:
  • Recombinant Kgp serves as a candidate antigen in developing vaccines to prevent P. gingivalis colonization and associated infections.
  • Used in immunogenicity studies to elicit protective immune responses.
• Structural and Biophysical Studies:
  • Facilitate crystallography or cryo-EM studies to determine the structure of Kgp and its interaction with inhibitors or host proteins.
  • Provide insights into the catalytic mechanism of lysine-specific proteolysis.

Kgp recombinant protein is an essential tool for understanding the virulence mechanisms of P. gingivalis and advancing periodontal disease research. Its applications in therapeutic development, diagnostics, and vaccine studies highlight its importance in addressing oral health challenges and combating bacterial infections.