KIAA recombinant proteins are derived from genes identified in the KIAA project, a large-scale effort to identify and sequence long cDNA clones encoding large proteins of unknown function in humans. These proteins are involved in various cellular processes and are often studied to elucidate their roles in cell signaling, structure, and disease. The KIAA nomenclature typically refers to proteins with uncertain or newly discovered functions, making them critical subjects in basic and translational research.
Content:
Structure and Function
- KIAA proteins are diverse in structure, often featuring domains associated with signaling, scaffolding, or enzymatic activity.
- Due to the exploratory nature of these proteins, recombinant forms may include the entire protein or specific functional domains.
- Functional studies often reveal roles in cell cycle regulation, apoptosis, signal transduction, or cytoskeletal organization.
Production
- KIAA recombinant proteins are produced in heterologous systems such as E. coli, yeast, insect cells, or mammalian cells depending on the protein's complexity.
- Expression tags (e.g., His-tag, GST-tag) are commonly used for purification, ensuring the production of soluble and active protein.
Applications
- These proteins are essential tools in functional genomics, used to identify the role of KIAA genes in various biological pathways.
- Employed in proteomics to identify interacting partners and post-translational modifications.
- Used in structural biology for crystallization and NMR studies, helping to determine the 3D structure of novel proteins.
Validation and Quality Control
- Recombinant KIAA proteins are validated through SDS-PAGE, mass spectrometry, and functional assays to ensure they meet research standards.
- Quality control ensures that the recombinant protein is correctly folded, active, and free of contaminants.
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