KLK Recombinant Proteins
KLK (Kallikrein) recombinant proteins are engineered versions of the serine proteases that belong to the kallikrein family. These proteins are involved in diverse physiological processes, including proteolysis, tissue remodeling, blood pressure regulation, and cancer progression.
Content on KLK Recombinant Proteins
- Structure and Function
- KLKs are secreted serine proteases with a characteristic catalytic triad (histidine, aspartate, and serine) in their active site.
- They are synthesized as inactive zymogens and activated via proteolytic cleavage.
- KLKs participate in:
- Proteolysis of extracellular matrix (ECM): Important in tissue remodeling and cancer metastasis.
- Kininogen cleavage: Releases kinins, which are vasoactive peptides regulating blood pressure and inflammation.
- Expression Systems
- Prokaryotic Systems (e.g., E. coli): Suitable for producing non-glycosylated forms or truncated versions.
- Eukaryotic Systems (e.g., insect or mammalian cells): Necessary for full-length KLKs with post-translational modifications (e.g., glycosylation) that are essential for activity.
Applications of KLK Recombinant Proteins
- Cancer Research
- Study KLKs as biomarkers for cancer diagnosis and prognosis, particularly KLK3 (prostate-specific antigen, PSA) for prostate cancer.
- Investigate their roles in tumor progression, invasion, and metastasis, especially in breast, ovarian, and pancreatic cancers.
- Protease Activity Studies
- Analyze the substrate specificity and catalytic mechanisms of KLKs.
- Explore their role in activating protease cascades involved in physiological and pathological conditions.
- Drug Discovery and Screening
- Develop inhibitors targeting KLK activity for therapeutic applications in cancer, inflammatory diseases, or hypertension.
- Screen for small molecules or peptides modulating KLK function in various signaling pathways.
KLK recombinant proteins are essential tools for advancing research into protease biology, cancer diagnostics, and therapeutic development, offering deep insights into their physiological and pathological roles.
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