KLK (Kallikrein) refers to a family of serine proteases involved in various physiological processes, including blood pressure regulation, tissue remodeling, and immune response. KLKs are characterized by their ability to cleave peptide bonds in proteins and are implicated in several diseases, including cancer and cardiovascular disorders.

Recombinant KLK Protein

Recombinant KLK proteins are produced using recombinant DNA technology. This process involves inserting the KLK gene into an expression vector, transforming it into a host cell system, and then expressing and purifying the protein.

Production Process
  • Gene Cloning: The gene encoding the specific KLK (e.g., KLK1, KLK3) is cloned into an expression vector, which may include tags to facilitate purification.
  • Transformation: The vector is introduced into a host cell system (e.g., E. coli, yeast, or mammalian cells) through techniques such as heat shock, electroporation, or transfection.
  • Expression: The host cells are cultured under conditions that induce the expression of the KLK protein.
  • Purification: The KLK protein is purified from the cell lysate or culture supernatant using chromatography techniques, such as affinity chromatography or ion exchange chromatography, to achieve high purity and functionality.
Structure and Function
  • Structure: KLKs are serine proteases with a typical structure including a catalytic domain that contains the active site where proteolytic activity occurs. They may also have additional domains depending on the specific KLK member.
  • Function: KLKs play diverse roles depending on the member. For example:
    • KLK1 (Kallikrein-1): Involved in the kininogen-kinin system, affecting blood pressure and fluid balance.
    • KLK3 (Prostate-Specific Antigen, PSA): Associated with prostate cancer, used as a biomarker for diagnosis and monitoring.
Applications
  • Research: Used to study protease activity, substrate specificity, and the physiological roles of KLKs in health and disease.
  • Drug Discovery: Facilitates the development of inhibitors targeting specific KLKs, which could be useful in treating diseases like cancer or hypertension.
  • Diagnostics: Recombinant KLKs, particularly KLK3 (PSA), are used in assays for cancer diagnostics and monitoring, especially in prostate cancer.
Quality Control
  • Purity: Verified by SDS-PAGE, Western blotting, and HPLC to ensure the protein is free from contaminants.
  • Activity: Confirmed through enzymatic assays to test the proteolytic activity and substrate specificity of the KLK protein.
  • Stability: Assessed under different storage conditions to ensure the protein maintains its functional integrity over time.

Recombinant KLK proteins are essential tools for understanding protease function, developing targeted therapies, and improving diagnostic assays. Their production and application span research into protease biology, drug discovery, and clinical diagnostics, contributing to advancements in medical science and treatment strategies.

517.00 517.0 USD
518.50 518.5 USD
517.00 517.0 USD
518.50 518.5 USD
517.00 517.0 USD
518.50 518.5 USD
518.50 518.5 USD
437.50 437.5 USD
437.50 437.5 USD
398.50 398.5 USD
398.50 398.5 USD
398.50 398.5 USD
398.50 398.5 USD
AffiREC® Recombinant Human KLK10 (C-6His)
CAT# AFG-EPR-0205
Size: 10 µg
237.15 237.15 USD