LPS Recombinant Proteins 

LPS (Lipopolysaccharide)-binding proteins are engineered proteins involved in the recognition and interaction with lipopolysaccharides, which are critical components of the outer membrane of Gram-negative bacteria. These proteins play key roles in innate immune responses, particularly in the detection of bacterial infections and modulation of inflammatory signaling.

Content on LPS Recombinant Proteins

• Structure and Function
  • LPS-binding proteins, such as LBP (Lipopolysaccharide-Binding Protein) or proteins containing LPS-recognizing motifs, mediate:
    • Recognition of LPS and its transfer to receptors like TLR4-MD2 complex.
    • Activation of innate immune signaling pathways, leading to cytokine production.
  • Domains in LPS-binding proteins are tailored for high-affinity binding to LPS molecules and initiation of downstream immune responses.
• Expression Systems
  • Prokaryotic Systems (e.g., E. coli): Used for producing non-glycosylated forms of LPS-recognizing domains.
  • Eukaryotic Systems (e.g., mammalian or insect cells): Necessary for full-length or glycosylated LPS-binding proteins with native functionality.

Applications of LPS Recombinant Proteins

• Infectious Disease Studies
  • Explore host-pathogen interactions, focusing on Gram-negative bacterial infections.
  • Study the role of LPS in sepsis and bacterial endotoxemia.
• Inflammatory Disease Research
  • Investigate the involvement of LPS-binding proteins in conditions like sepsis, chronic inflammation, and autoimmunity.
  • Use recombinant proteins to study cytokine release and immune activation in response to bacterial components.
• Drug Discovery and Therapeutics
  • Screen inhibitors targeting LPS recognition and signaling pathways to develop anti-inflammatory or anti-sepsis drugs.
  • Test the efficacy of LPS-neutralizing compounds or antibodies.

LPS recombinant proteins are invaluable tools in studying bacterial infections, immune activation, and inflammation, contributing to advancements in diagnostics, therapeutics, and vaccine development.