LRP Recombinant Proteins
Low-Density Lipoprotein Receptor-Related Proteins (LRPs) are a family of multifunctional receptors involved in endocytosis, signal transduction, and cell homeostasis. LRP proteins, including LRP1, LRP2 (megalin), and others, play pivotal roles in lipid metabolism, clearance of apoptotic cells, and cellular signaling pathways such as Wnt and TGF-β. Dysregulation of LRP functions is associated with diseases such as atherosclerosis, neurodegeneration, and cancer.
Content of LRP Recombinant Proteins
- Production:
- Produced in mammalian or insect cell systems to ensure proper folding and glycosylation.
- Purification achieved through affinity and size-exclusion chromatography.
- Variants:
- Full-length proteins for receptor-ligand interaction studies.
- Domain-specific constructs (e.g., ligand-binding domains, transmembrane segments) for mechanistic insights.
- Mutant forms for investigating receptor functionality or signaling defects.
- Specifications:
- Purity: >90% as verified by SDS-PAGE and HPLC.
- Bioactivity: Validated via binding assays or ligand internalization studies.
- Formulation: Stabilized in Tris-buffered saline or phosphate buffers with cryoprotectants.
Applications of LRP Recombinant Proteins
- Drug Discovery and Screening:
- High-throughput screening of LRP-targeting drugs or biologics, such as antibodies or small molecules, for therapeutic interventions in cancer or Alzheimer’s disease.
- Identification of LRP modulators for controlling lipid metabolism in cardiovascular diseases.
- Structural Biology:
- Facilitates structural determination through X-ray crystallography or cryo-EM to understand ligand-binding and activation mechanisms.
- Used for mapping drug-binding pockets for structure-guided drug design.
- Biomarker Discovery:
- Recombinant LRP proteins serve as references for ELISA or other immunoassays in biomarker development for neurodegenerative diseases like Alzheimer’s.
- Aid in generating high-specificity antibodies against LRP domains.
LRP recombinant proteins are crucial for advancing our understanding of their roles in cellular and systemic functions. They support diverse research areas, including receptor biology, drug discovery, and biomarker development, making them indispensable tools in modern biomedical research.
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