LRRC Recombinant Proteins 

LRRC recombinant proteins are synthetic versions of Leucine-Rich Repeat-Containing (LRRC) proteins, which are a family of proteins characterized by the presence of leucine-rich repeat (LRR) motifs. These motifs mediate protein-protein interactions and are crucial in various cellular processes, including signal transduction, cell adhesion, and immune responses.

Technical Content

• Expression Systems:
  • Mammalian cells (HEK293, CHO): Preferred for full-length proteins requiring glycosylation or other post-translational modifications.
  • E. coli: Suitable for truncated domains or non-glycosylated forms of LRRC proteins.
  • Insect cells: Used for complex or large LRRC proteins requiring proper folding and dimerization.
• Purification and Validation:
  • Purification involves affinity chromatography (e.g., His-tag, GST-tag), followed by size-exclusion chromatography to ensure structural homogeneity.
  • Quality control:
    • SDS-PAGE and Western blotting for purity and size validation.
    • ELISA or functional assays to confirm activity.
    • Structural integrity validated using mass spectrometry and circular dichroism (CD).
• Variants and Constructs:
  • Recombinant LRRC proteins are available as:
    • Full-length proteins for structural and interaction studies.
    • Functional domains (e.g., extracellular LRR regions) for ligand binding assays.

Applications of LRRC Recombinant Proteins

• Signal Transduction Studies:
  • LRRC8A: Investigating the role of VRAC in cellular osmoregulation and apoptosis.
  • LRRC15: Studying its involvement in modulating immune responses and its role as a therapeutic target in cancer immunotherapy.
• Neuroscience Research:
  • LRRC4B: Understanding its role in synapse formation, neuronal connectivity, and neurodevelopmental disorders.
  • Recombinant LRRC proteins are used to study protein-protein interactions critical in brain signaling pathways.
• Cancer Research:
  • LRRC15: Implicated in tumor progression and resistance to immune checkpoint therapies, making it a target for cancer research and drug discovery.
  • Used in drug screening assays for inhibitors or monoclonal antibodies targeting LRRC15-mediated pathways.

LRRC recombinant proteins are indispensable tools for studying the diverse biological functions of leucine-rich repeat-containing proteins. They are pivotal in elucidating the molecular mechanisms underlying signal transduction, immune regulation, and neural connectivity.