MMP Recombinant Proteins
MMP (Matrix Metalloproteinase) recombinant proteins are engineered forms of a family of zinc-dependent endopeptidases that play a critical role in extracellular matrix (ECM) remodeling. MMPs are involved in tissue repair, angiogenesis, embryogenesis, and immune responses, as well as pathological conditions like cancer metastasis, arthritis, and cardiovascular diseases.
Content on MMP Recombinant Proteins
- Structure and Function
- MMPs consist of multiple domains:
- Pro-domain: Keeps the enzyme inactive until cleavage.
- Catalytic Domain: Contains the active site with a zinc ion critical for proteolytic activity.
- Hemopexin-like Domain (in some MMPs): Facilitates substrate specificity and interactions with ECM components.
- MMPs are classified into groups based on substrate specificity, such as:
- Collagenases (e.g., MMP-1, MMP-8): Degrade fibrillar collagens.
- Gelatinases (e.g., MMP-2, MMP-9): Act on denatured collagens and other ECM components.
- Stromelysins (e.g., MMP-3, MMP-10): Degrade proteoglycans, laminin, and other non-collagen substrates.
- MMPs consist of multiple domains:
- Expression Systems
- Prokaryotic Systems (e.g., E. coli): Suitable for producing truncated or catalytic domains of MMPs.
- Eukaryotic Systems (e.g., insect or mammalian cells): Necessary for full-length, glycosylated MMPs with proper folding and activation.
Applications of MMP Recombinant Proteins
- Cancer Research
- Study the role of MMPs in tumor invasion, metastasis, and angiogenesis.
- Explore MMP inhibition as a therapeutic strategy for limiting cancer progression.
- Inflammatory and Autoimmune Diseases
- Examine MMP roles in chronic inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease (IBD).
- Study their contribution to tissue damage in autoimmune conditions.
- Cardiovascular Research
- Explore MMP activity in vascular remodeling, atherosclerosis, and aneurysm formation.
- Investigate their role in myocardial remodeling following ischemic injury.
MMP recombinant proteins are critical for understanding the roles of matrix remodeling in physiology and pathology, offering insights into therapeutic strategies and biomarker discovery for a wide range of diseases.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|