MonkeyPox Recombinant Proteins 

Monkeypox recombinant proteins are engineered proteins derived from key components of the Monkeypox virus (MPXV), an orthopoxvirus closely related to smallpox. These proteins are typically expressed in vitro to mimic native viral proteins and are crucial tools for studying viral pathogenesis, immune responses, diagnostic assay development, and vaccine research. 

Content on Monkeypox Recombinant Proteins

Monkeypox recombinant proteins are synthesized using advanced expression systems such as E. coli, baculovirus-insect cells, or mammalian cells to ensure proper folding, post-translational modifications, and biological functionality. Key features include:

• Variants:
  • A35R: Immunogenic protein used in vaccine and immune response studies.
  • B6R and H3L: Envelope proteins critical for viral entry and assembly.
  • D8L and A27L: Structural proteins important for virus-host interactions.
• Purity: Typically >95%, validated using SDS-PAGE and HPLC.
• Bioactivity: Retains native properties for binding assays and serological applications.

Applications of Monkeypox Recombinant Proteins

• Serological Diagnostics:
  • Used as antigens in ELISA, Western blot, and immunofluorescence assays to detect anti-Monkeypox virus antibodies in clinical samples.
  • Incorporated as standards or controls in diagnostic kits for identifying MPXV infections.
• Vaccine Research:
  • Supports the design and testing of candidate vaccines by studying immune responses to specific viral proteins.
  • Evaluates immunogenicity and efficacy in preclinical and clinical studies.
• Drug Development:
  • Facilitates screening and validation of antiviral compounds targeting Monkeypox virus proteins.
  • Used in functional assays to assess inhibition of viral entry, replication, and assembly.

Monkeypox recombinant proteins are invaluable for advancing research into Monkeypox virus pathogenesis, diagnostics, and therapeutics. Their high-quality production ensures reproducibility and reliability, making them essential tools in virology, immunology, and public health.